PMID- 7607551
OWN - NLM
STAT- MEDLINE
DCOM- 19950815
LR  - 20231213
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 158
IP  - 2
DP  - 1995 Jun 9
TI  - Use of alternate promoters for tissue-specific expression of the gene coding for 
      connexin32.
PG  - 257-62
AB  - The promoter of rat connexin32 (Cx32), the gap junction protein found in liver, 
      was studied in transgenic mice. Cx32 transgenes, containing 2.5-kb of sequence 
      upstream from the promoter, exon I, the entire 6.1-kb intron and the beginning of 
      the coding sequence linked to the gene encoding luciferase (Luc), were found to 
      be expressed in mouse in the same tissue-specific manner as previously reported 
      for Cx32. Another construct lacking the promoter, but retaining 1.8 kb from the 
      3' end of the intron, was found to be expressed specifically in the nervous 
      system. This result suggested that a second promoter, different from that used in 
      liver, functions in nervous tissue. The use of this promoter in normal rats was 
      corroborated by sequence analysis of reverse-transcribed PCR products obtained 
      from rat nervous tissue RNA. The second promoter drives the synthesis of a second 
      Cx32 mRNA species that is processed to remove a small 345-bp intron that shares 
      its acceptor splice site with the large intron. This finding could have 
      implications for the genetic basis of the X-linked form of Charcot-Marie-Tooth 
      disease (CMT-X) in those patients that do not exhibit mutations in the 
      Cx32-coding region.
FAU - Neuhaus, I M
AU  - Neuhaus IM
AD  - Department of Biochemistry, University of Miami, School of Medicine, FL 33101, 
      USA.
FAU - Dahl, G
AU  - Dahl G
FAU - Werner, R
AU  - Werner R
LA  - eng
SI  - GENBANK/L36875
SI  - GENBANK/L36876
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Connexins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Connexins/*genetics/isolation & purification
MH  - Fluorescent Antibody Technique
MH  - *Gene Expression Regulation
MH  - Liver/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Nervous System/metabolism
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Tissue Distribution
MH  - Gap Junction beta-1 Protein
EDAT- 1995/06/09 00:00
MHDA- 1995/06/09 00:01
CRDT- 1995/06/09 00:00
PHST- 1995/06/09 00:00 [pubmed]
PHST- 1995/06/09 00:01 [medline]
PHST- 1995/06/09 00:00 [entrez]
AID - 0378111994008994 [pii]
AID - 10.1016/0378-1119(94)00899-4 [doi]
PST - ppublish
SO  - Gene. 1995 Jun 9;158(2):257-62. doi: 10.1016/0378-1119(94)00899-4.