PMID- 7607652
OWN - NLM
STAT- MEDLINE
DCOM- 19950817
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 96
IP  - 1
DP  - 1995 Jul
TI  - Identification of the origin of double minutes in normal human cells by 
      laser-based chromosome microdissection approach.
PG  - 39-43
AB  - Single copies of tiny chromosome fragments, appearing as double minutes, were 
      observed in a high proportion of cells from amniotic fluid cultures of two 
      mothers undergoing prenatal testing because of advanced age. We applied a 
      laser-based chromosome microdissection method to diagnose the origin of the 
      double minutes. The diagnostic procedures consisted of microdissection of double 
      minutes from a single cell, polymerase chain reaction (PCR) amplification of the 
      dissected DNA, and subsequent fluorescence in situ hybridization (FISH) using the 
      PCR products as a probe pool. Metaphase chromosomes from the patients' cells and 
      from a karyotypically normal individual were probed. Using this strategy, we were 
      able to determine that the double minutes originated from the centromere of 
      chromosome 13 or 21 in one case, and from the chromosome 12 centromere in the 
      other. The characterization of such double minutes helps both in the delineation 
      of the nature of these epichromosomal bodies in normal individuals as well as in 
      the clarification of genetic counselling issues.
FAU - Rajcan-Separovic, E
AU  - Rajcan-Separovic E
AD  - GenoSPHERE Project, University of Ottawa, Faculty of Medicine, Ontario, Canada.
FAU - Wang, H S
AU  - Wang HS
FAU - Speevak, M D
AU  - Speevak MD
FAU - Janes, L
AU  - Janes L
FAU - Korneluk, R G
AU  - Korneluk RG
FAU - Wakasa, K
AU  - Wakasa K
FAU - Ikeda, J E
AU  - Ikeda JE
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (Chromatin)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromatin/*genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 21
MH  - DNA Probes
MH  - Dissection
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Karyotyping
MH  - *Lasers
MH  - Polymerase Chain Reaction
MH  - Reference Values
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1007/BF00214184 [doi]
PST - ppublish
SO  - Hum Genet. 1995 Jul;96(1):39-43. doi: 10.1007/BF00214184.