PMID- 7608776
OWN - NLM
STAT- MEDLINE
DCOM- 19950817
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 73
IP  - 3
DP  - 1995 Mar
TI  - Pancreatic polypeptide inhibits calcium channels in rat sympathetic neurons via 
      two signaling pathways.
PG  - 1323-8
AB  - 1. We studied modulation of N-type Ca2+ channels in adult rat superior cervical 
      ganglion (SCG) neurons by pancreatic polypeptide (PP) using whole cell clamp. In 
      large (> 20 pF) SCG neurons, PP inhibited ICa (35 +/- 2%, mean +/- SE) in a 
      concentration-dependent fashion, with one-half maximal inhibition at 19 nM. 2. 
      One-third of the inhibition was blocked by pertussis toxin, about one-half was 
      blocked by N-ethylmaleimide (NEM) treatments, and about one-half was voltage 
      dependent. The NEM-insensitive component of the PP inhibition was voltage 
      independent and not significantly blocked by intracellular Ca2+ chelators. 3. The 
      NEM-insensitive component was only weakly attenuated by GDP-beta-S, and 
      moderately reversible with guanosine 5'-triphosphate (GTP)-gamma-S, in the whole 
      cell pipette, leaving open the possibility that it is not mediated by a G 
      protein. 4. Hence, PP inhibits ICa via two mechanisms: one G-protein-mediated and 
      the other possibly G-protein independent. The former pathway is sensitive to 
      pertussis toxin (PTX) and NEM, voltage dependent, and shared by several other 
      transmitters in these cells. The latter pathway is PTX-and NEM-insensitive, not 
      voltage dependent, and not affected by the presence of intracellular Ca2+ 
      chelators.
FAU - Wollmuth, L P
AU  - Wollmuth LP
AD  - Department of Physiology and Biophysics, University of Washington, Seattle 98195, 
      USA.
FAU - Shapiro, M S
AU  - Shapiro MS
FAU - Hille, B
AU  - Hille B
LA  - eng
GR  - NS-07097/NS/NINDS NIH HHS/United States
GR  - NS-08174/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Calcium Channels)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 59763-91-6 (Pancreatic Polypeptide)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
SB  - IM
MH  - Adrenergic Fibers/*drug effects
MH  - Animals
MH  - Calcium Channels/*drug effects
MH  - Cells, Cultured/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Pancreatic Polypeptide/*pharmacology
MH  - Pertussis Toxin
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Time Factors
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1152/jn.1995.73.3.1323 [doi]
PST - ppublish
SO  - J Neurophysiol. 1995 Mar;73(3):1323-8. doi: 10.1152/jn.1995.73.3.1323.