PMID- 7609648
OWN - NLM
STAT- MEDLINE
DCOM- 19950817
LR  - 20190825
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 30
IP  - 1
DP  - 1995 May
TI  - Expression of cholecystokinin mRNA in corticothalamic projecting neurons: a 
      combined fluorescence in situ hybridization and retrograde tracing study in the 
      ventrolateral thalamus of the rat.
PG  - 87-96
AB  - Cholecystokinin (CCK), a well-known neuroactive peptide, has been observed in the 
      axon endings within the thalamic reticular nucleus and the adjacent ventrolateral 
      nucleus of the thalamus. The origin of this CCK innervation remains undefined. In 
      this study, a fluorescence in situ hybridization (FISH) technique was used in 
      conjunction with latex microsphere retrograde tracing to investigate whether 
      cortical neurons may provide a source of CCK afferents to the ventrolateral 
      thalamic nucleus. Rhodamine latex beads were injected into the ventrolateral 
      thalamic nucleus of adult male rats to retrogradely label corticothalamic cells. 
      After 7 days, tissues were processed for FISH using a 24-base oligonucleotide 
      probe complementary to the 3' coding region of rat preprocholecystokinin mRNA. It 
      was found that CCK transcripts are expressed in about 80% of identified 
      corticothalamic projecting neurons. We therefore conclude that the descending 
      cortical projections to the ventrolateral thalamus may provide an important 
      source of CCK innervation to this region of the brain.
FAU - Senatorov, V V
AU  - Senatorov VV
AD  - Neuroscience Unit, Loeb Research Institute, Ottawa Civic Hospital/University of 
      Ottawa, Ont., Canada.
FAU - Trudeau, V L
AU  - Trudeau VL
FAU - Hu, B
AU  - Hu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (RNA, Messenger)
RN  - 9011-97-6 (Cholecystokinin)
SB  - IM
MH  - Animals
MH  - Cerebral Cortex/metabolism
MH  - Cholecystokinin/*biosynthesis/genetics
MH  - Fluorescence
MH  - Gene Expression
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Neural Pathways/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Thalamus/*metabolism
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 0169328X9400282J [pii]
AID - 10.1016/0169-328x(94)00282-j [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1995 May;30(1):87-96. doi: 10.1016/0169-328x(94)00282-j.