PMID- 7611178
OWN - NLM
STAT- MEDLINE
DCOM- 19950817
LR  - 20190512
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 104
IP  - 1
DP  - 1995 Jul
TI  - Identification of male epithelial cells in routine postcoital cervicovaginal 
      smears using fluorescence in situ hybridization. Application in sexual assault 
      and molestation.
PG  - 32-5
AB  - In a prospective blinded controlled study, the efficacy of fluorescence in situ 
      hybridization (FISH) to detect non-sperm male cells verifying sexual contact was 
      examined. Cervicovaginal smears (CVS) from 40 women with reported post-coital 
      intervals were examined for sperm by cytology and for sperm and non-sperm male 
      cells by FISH using X and Y chromosome specific DNA probes. Fluorescence in situ 
      hybridization identified sperm and/or non-sperm male cells in all specimens from 
      women with positive coital histories, including when the partner had a vasectomy. 
      Male cells were also detectable by FISH in CVS up to 3 weeks after coitus. In 
      comparison, cytology identified sperm in 41% of the positive coital history 
      cases, and none beyond 2 weeks. Fluorescence in situ hybridization is highly 
      sensitive and specific in detecting male cells, and can be performed rapidly on 
      routine CVS. Application of this technique can provide new and additional 
      evidence of sexual contact when current tests are inconclusive.
FAU - Roa, P N
AU  - Roa PN
AD  - Department of Pediatrics, Bowman Gray School of Medicine of Wake Forest 
      University, Winston-Salem, North Carolina 27157, USA.
FAU - Collins, K A
AU  - Collins KA
FAU - Geisinger, K R
AU  - Geisinger KR
FAU - Parsons, L H
AU  - Parsons LH
FAU - Schnell, S
AU  - Schnell S
FAU - Hayworth-Hodge, R
AU  - Hayworth-Hodge R
FAU - Tap, M P
AU  - Tap MP
FAU - Lantz, P E
AU  - Lantz PE
FAU - Pettenati, M J
AU  - Pettenati MJ
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Coitus
MH  - DNA Probes
MH  - Double-Blind Method
MH  - Epithelial Cells
MH  - Epithelium/ultrastructure
MH  - Female
MH  - Genitalia, Male/*cytology/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*standards
MH  - Male
MH  - Prospective Studies
MH  - Rape/*legislation & jurisprudence
MH  - Spermatozoa/cytology/ultrastructure
MH  - Vagina/*cytology
MH  - Vaginal Smears/*standards
MH  - X Chromosome/ultrastructure
MH  - Y Chromosome/ultrastructure
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1093/ajcp/104.1.32 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1995 Jul;104(1):32-5. doi: 10.1093/ajcp/104.1.32.