PMID- 7611452
OWN - NLM
STAT- MEDLINE
DCOM- 19950814
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 268
IP  - 6 Pt 2
DP  - 1995 Jun
TI  - Regulation of renal growth factors and clusterin by AT1 receptors during neonatal 
      ureteral obstruction.
PG  - F1117-23
AB  - Unilateral ureteral obstruction (UUO) in the neonate impairs growth of the 
      ipsilateral kidney. Since renal renin expression is increased by UUO, we 
      hypothesized that, by activation of AT1 receptors, angiotensin II (ANG II) 
      regulates expression of transforming growth factor-beta 1 (TGF-beta 1) and 
      epidermal growth factor (EGF) in the obstructed kidney. Sprague-Dawley rats 
      underwent left UUO or sham operation within the first 48 h of life and received 
      losartan, 40 mg.kg-1.day-1, or saline. After 14 days, steady-state renal mRNA was 
      determined for renin, TGF-beta 1, EGF, and clusterin. Losartan reduced the DNA 
      content of the intact kidneys but did not further decrease that of the obstructed 
      kidney. Losartan increased renal renin expression and decreased EGF expression by 
      80%, regardless of UUO. In contrast, losartan reduced TGF-beta 1 expression by 
      34% in obstructed kidneys but did not affect TGF-beta 1 in intact kidneys. 
      Losartan increased clusterin expression by 60% in obstructed kidneys and 
      seven-fold in intact kidneys. We conclude that activation of the ANG II AT1 
      receptor is necessary for normal renal growth and that TGF-beta 1 is regulated by 
      AT1 receptors in the obstructed, but not intact, kidneys. Through AT1 receptors, 
      endogenous ANG II stimulates EGF and inhibits clusterin expression.
FAU - Chung, K H
AU  - Chung KH
AD  - Department of Urology, Gyeong-sang National University, Chinju, Korea.
FAU - Gomez, R A
AU  - Gomez RA
FAU - Chevalier, R L
AU  - Chevalier RL
LA  - eng
GR  - DK-44756/DK/NIDDK NIH HHS/United States
GR  - DK-45179/DK/NIDDK NIH HHS/United States
GR  - HD-28810/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Angiotensin Receptor Antagonists)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Clusterin)
RN  - 0 (Glycoproteins)
RN  - 0 (Imidazoles)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Angiotensin)
RN  - 0 (Tetrazoles)
RN  - 0 (Transforming Growth Factor beta)
RN  - 11128-99-7 (Angiotensin II)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 3.4.23.15 (Renin)
RN  - JMS50MPO89 (Losartan)
SB  - IM
MH  - Angiotensin II/antagonists & inhibitors/*pharmacology
MH  - Angiotensin Receptor Antagonists
MH  - Animals
MH  - Animals, Newborn
MH  - Biphenyl Compounds/pharmacology
MH  - Blotting, Northern
MH  - Clusterin
MH  - DNA/analysis/metabolism
MH  - Epidermal Growth Factor/*biosynthesis
MH  - Gene Expression/drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis
MH  - Glycoproteins/*biosynthesis
MH  - Imidazoles/pharmacology
MH  - Kidney/*metabolism/physiopathology
MH  - Losartan
MH  - *Molecular Chaperones
MH  - Nerve Tissue Proteins/biosynthesis
MH  - RNA, Messenger/analysis/biosynthesis/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Angiotensin/*physiology
MH  - Reference Values
MH  - Renin/biosynthesis
MH  - Tetrazoles/pharmacology
MH  - Transforming Growth Factor beta/*biosynthesis
MH  - Ureteral Obstruction/*physiopathology
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1152/ajprenal.1995.268.6.F1117 [doi]
PST - ppublish
SO  - Am J Physiol. 1995 Jun;268(6 Pt 2):F1117-23. doi: 
      10.1152/ajprenal.1995.268.6.F1117.