PMID- 7611789
OWN - NLM
STAT- MEDLINE
DCOM- 19950815
LR  - 20061115
IS  - 0385-0684 (Print)
IS  - 0385-0684 (Linking)
VI  - 22 Suppl 2
DP  - 1995 Jun
TI  - [Detection of chromosomal aberration using fluorescence in situ hybridization on 
      breast cancer].
PG  - 192-6
AB  - The relationship between interphase cytogenetics and prognostic factors, 
      especially the grade of nuclear atypism, nuclear DNA content, histological lymph 
      node metastasis and clinical data was examined in 48 primary breast cancer 
      specimens (touch preparation). Using fluorescence in situ hybridization (FISH) 
      with a chromosome-specific DNA probe, the copy number of pericentrometric 
      sequences on chromosome 17 was examined within the interphase nuclei in touch 
      preparations from the tumor. The copy number of chromosome 17 was correlated with 
      the increase in the grade of nuclear atypism, tumor size, histological lymph node 
      metastasis and nuclear DNA content. In the diploid type of nuclear DNA content, 
      the copy number of chromosome 17 was correlated with the increase in the grade of 
      nuclear atypism and histological lymph node metastasis. In conclusion, the 
      numerical chromosomal aberrations detected by FISH were found in the DNA diploid 
      cases by FCM. The detection of numerical chromosomal aberrations by FISH provide 
      important information about the prognostic factors.
FAU - Iwamoto, S
AU  - Iwamoto S
AD  - Dept. of Surgery, Osaka Medical College.
FAU - Okajima, K
AU  - Okajima K
FAU - Ryo, T
AU  - Ryo T
FAU - Ishibashi, T
AU  - Ishibashi T
FAU - Toyoda, M
AU  - Toyoda M
FAU - Ichinona, T
AU  - Ichinona T
FAU - Osada, K
AU  - Osada K
FAU - Gon, G
AU  - Gon G
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Gan To Kagaku Ryoho
JT  - Gan to kagaku ryoho. Cancer & chemotherapy
JID - 7810034
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphatic Metastasis
MH  - Ploidies
MH  - Trisomy
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
PST - ppublish
SO  - Gan To Kagaku Ryoho. 1995 Jun;22 Suppl 2:192-6.