PMID- 7616606
OWN - NLM
STAT- MEDLINE
DCOM- 19950823
LR  - 20071114
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 40
IP  - 4
DP  - 1995 Mar 1
TI  - Effects of carbonic anhydrase II (CAII) deficiency on CNS structure and function 
      in the myelin-deficient CAII-deficient double mutant mouse.
PG  - 451-7
AB  - In the choroid plexus carbonic anhydrase II (CAII) supports the transport of 
      bicarbonate ions, sodium ions, and water from blood to the CSF, and in the myelin 
      sheath CAII supports compaction of myelin by stimulating cotransport of ions and 
      water out from between the myelin membranes. In view of the latter, it is 
      surprising that mutant mice deficient in CAII (Car-2n) have compact myelin. Since 
      myelin basic protein also takes part in myelin compaction, we bred double 
      CAII-deficient, myelin-deficient (Mld) mutant mice, in which the adults would 
      have some compact myelin sheaths and a partial deficiency in myelin basic 
      protein, with a view to examining oligodendrocytes and myelin sheaths in the 
      double mutant. Like the parent Mld strain, the double mutants displayed tremors 
      and seizures; however, the onset of seizures was delayed significantly in the 
      double mutants, and the lifespan increased by several months. Like the brains of 
      Car-2n mutants, those of double mutants (MldCar-2n) were deficient in mRNA and 
      protein for CAII and showed upregulation of a different isozyme, CAIV. In the 
      double mutants, oligodendrocytes were reduced in number, and the myelin sheaths 
      and oligodendrocytes were swollen. The partial protection against seizures, which 
      CAII deficiency conferred, suggests that acidosis in the central nervous system 
      (CNS) of the Car-2n and MldCar-2n mice, due to absence of CAII from the choroid 
      plexus, may downregulate the activity of NMDA receptors.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Cammer, W
AU  - Cammer W
AD  - Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 
      10461, USA.
FAU - Zhang, H
AU  - Zhang H
FAU - Tansey, F A
AU  - Tansey FA
LA  - eng
GR  - NS 12890/NS/NINDS NIH HHS/United States
GR  - NS 23705/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Myelin Proteins)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Carbonic Anhydrases/*deficiency/*genetics
MH  - Central Nervous System/*enzymology
MH  - Corpus Striatum/immunology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Myelin Proteins/*ultrastructure
MH  - Neuroglia
MH  - Oligodendroglia
MH  - Prosencephalon
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1002/jnr.490400404 [doi]
PST - ppublish
SO  - J Neurosci Res. 1995 Mar 1;40(4):451-7. doi: 10.1002/jnr.490400404.