PMID- 7616958
OWN - NLM
STAT- MEDLINE
DCOM- 19950822
LR  - 20201209
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 247
IP  - 6
DP  - 1995 Jun 25
TI  - Sequencing and analysis of the gene encoding the alpha-toxin of Clostridium novyi 
      proves its homology to toxins A and B of Clostridium difficile.
PG  - 670-9
AB  - A library of total Clostridium novyi DNA was established and screened for the 
      alpha-toxin gene (tcn alpha) by hybridization with oligonucleotides derived from 
      a partial N-terminal sequence and by using specific antisera. Overlapping 
      subgenic tcn alpha fragments were isolated and subsequently the total sequence of 
      tcn alpha was determined. The 6534 nucleotide open reading frame encodes a 
      polypeptide of M(r) 250,166 and pI 5.9. The N-terminal alpha-toxin (Tcn alpha) 
      sequence MLITREQLMKIASIP determined by Edman degradation confirmed the identity 
      of the reading frame and the assignment of the translation start point. The toxin 
      is not modified posttranslationally at its N-terminus nor does it consist of 
      different subunits. Overall the amino acid sequence shows 48% homology between 
      the Tcn alpha and both toxins A (TcdA) and B (TcdB) of Clostridium difficile. The 
      C-terminal 382 residues of Tcn alpha constitute a repetitive domain similar to 
      those reported for TcdA and TcdB of C. difficile. The individual repeat motifs of 
      these three toxins consist of oligopeptides some 19-52 amino acids in length, 
      arranged in four to five different groups. Genetic, biochemical and 
      pharmacological data thus confirm that the three toxins belong to one subgroup, 
      designated large clostridial cytotoxins (LCT). Further definition of their 
      structure and detailed molecular action should allow the LCTs to be used tools 
      for the analysis of microfilament assembly and function.
FAU - Hofmann, F
AU  - Hofmann F
AD  - Institut fur Medizinische Mikrobiologie, Johannes Gutenberg-Universitat Mainz, 
      Germany.
FAU - Herrmann, A
AU  - Herrmann A
FAU - Habermann, E
AU  - Habermann E
FAU - von Eichel-Streiber, C
AU  - von Eichel-Streiber C
LA  - eng
SI  - GENBANK/Z48636
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (tcdA protein, Clostridium difficile)
RN  - 0 (toxB protein, Clostridium difficile)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
GS  - tcn&agr;
MH  - Amino Acid Sequence
MH  - *Bacterial Proteins
MH  - Bacterial Toxins/chemistry/genetics
MH  - Base Sequence
MH  - Clostridioides difficile/genetics
MH  - Clostridium/*genetics
MH  - Enterotoxins/chemistry/genetics
MH  - Genomic Library
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/chemistry/genetics
MH  - Sequence Homology, Amino Acid
MH  - Type C Phospholipases/chemistry/*genetics
EDAT- 1995/06/25 00:00
MHDA- 1995/06/25 00:01
CRDT- 1995/06/25 00:00
PHST- 1995/06/25 00:00 [pubmed]
PHST- 1995/06/25 00:01 [medline]
PHST- 1995/06/25 00:00 [entrez]
AID - 10.1007/BF00290398 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1995 Jun 25;247(6):670-9. doi: 10.1007/BF00290398.