PMID- 7618083
OWN - NLM
STAT- MEDLINE
DCOM- 19950822
LR  - 20211203
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 269
IP  - 5221
DP  - 1995 Jul 14
TI  - Regulation of human leukocyte p21-activated kinases through G protein--coupled 
      receptors.
PG  - 221-3
AB  - The Rac guanosine 5'-triphosphate (GTP)-binding proteins regulate oxidant 
      production by phagocytic leukocytes. Two Ste20-related p21-activated kinases 
      (PAKs) were identified as targets of Rac in human neutrophils. Activity of the 
      approximately 65- and approximately 68-kilodalton PAKs was rapidly stimulated by 
      chemoattractants acting through pertussis toxin-sensitive heterotrimeric 
      GTP-binding proteins (G proteins). Native and recombinant PAKs phosphorylated the 
      p47phox reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 
      component in a Rac-GTP-dependent manner. The action of PAKs during phagocyte 
      activation by G protein-coupled pathways may contribute to regulation of NADPH 
      oxidase activity.
FAU - Knaus, U G
AU  - Knaus UG
AD  - Department of Immunology, Scrippts Research Institute, La Jolla, CA 92037, USA.
FAU - Morris, S
AU  - Morris S
FAU - Dong, H J
AU  - Dong HJ
FAU - Chernoff, J
AU  - Chernoff J
FAU - Bokoch, G M
AU  - Bokoch GM
LA  - eng
GR  - AI35947/AI/NIAID NIH HHS/United States
GR  - HL48008/HL/NHLBI NIH HHS/United States
GR  - P60 AR40770/AR/NIAMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.1 (neutrophil cytosolic factor 1)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 2.7.11.1 (PAK1 protein, human)
RN  - EC 2.7.11.1 (PAK2 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Enzyme Activation
MH  - GTP-Binding Proteins/*metabolism
MH  - Guanosine Triphosphate/*metabolism
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - NADH, NADPH Oxidoreductases/metabolism
MH  - NADPH Dehydrogenase/metabolism
MH  - NADPH Oxidases
MH  - Neutrophil Activation
MH  - Neutrophils/*enzymology
MH  - Pertussis Toxin
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Virulence Factors, Bordetella/pharmacology
MH  - p21-Activated Kinases
MH  - rac GTP-Binding Proteins
EDAT- 1995/07/14 00:00
MHDA- 1995/07/14 00:01
CRDT- 1995/07/14 00:00
PHST- 1995/07/14 00:00 [pubmed]
PHST- 1995/07/14 00:01 [medline]
PHST- 1995/07/14 00:00 [entrez]
AID - 10.1126/science.7618083 [doi]
PST - ppublish
SO  - Science. 1995 Jul 14;269(5221):221-3. doi: 10.1126/science.7618083.