PMID- 7619360
OWN - NLM
STAT- MEDLINE
DCOM- 19950831
LR  - 20190512
IS  - 0895-7061 (Print)
IS  - 0895-7061 (Linking)
VI  - 8
IP  - 4 Pt 1
DP  - 1995 Apr
TI  - Intrarenal arterial network renin content and inhibition by EMD 58265.
PG  - 433-6
AB  - The renin content, expressed as nanograms of angiotensin I/hour/milligram, of a 
      rabbit intrarenal arterial network (IAN) was compared to that of renal cortical 
      samples from the same animal. Tissues from six rabbits were studied. Cortical 
      tissue (RC) and an IAN with some glomeruli and attached arterioles (IAN + 
      glomeruli) were prepared from one kidney, and an IAN freed of glomeruli (IAN - 
      glomeruli) was obtained from the opposite kidney. These tissues were homogenized 
      and the supernatant incubated at pH 7.4 and 37 degrees C for 30 to 60 min or 3 to 
      6 h. Angiotensin I was generated in a linear fashion, and the values averaged for 
      the two incubation periods were 844 +/- 266, 121 +/- 39, and 32 +/- 11 ng/h/mg 
      for RC, IAN + glomeruli, and IAN - glomeruli, respectively. A specific renin 
      inhibitor, EMD 58265, at 1 x 10(-8) mol/L attenuated and at 5 x 10(-8) mol/L 
      almost totally abolished angiotensin I production. The results indicate that 
      small arteries comprising the IAN contain renin, and that the enzymatic activity 
      is most likely due to that protein and not another aspartyl protease.
FAU - Birt, P C
AU  - Birt PC
AD  - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, 
      USA.
FAU - Zimmerman, B G
AU  - Zimmerman BG
LA  - eng
GR  - HL 39698/HL/NHLBI NIH HHS/United States
GR  - HL 44370/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Hypertens
JT  - American journal of hypertension
JID - 8803676
RN  - 0 (EMD 58265)
RN  - 0 (Oligopeptides)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - Animals
MH  - Juxtaglomerular Apparatus/drug effects/metabolism
MH  - Kidney Cortex/drug effects/metabolism
MH  - Oligopeptides/*pharmacology
MH  - Rabbits
MH  - Renal Artery/*metabolism
MH  - Renin/*antagonists & inhibitors/*blood
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 089570619400073K [pii]
AID - 10.1016/0895-7061(94)00073-k [doi]
PST - ppublish
SO  - Am J Hypertens. 1995 Apr;8(4 Pt 1):433-6. doi: 10.1016/0895-7061(94)00073-k.