PMID- 7620643 OWN - NLM STAT- MEDLINE DCOM- 19950831 LR - 20190909 IS - 1045-3873 (Print) IS - 1045-3873 (Linking) VI - 6 IP - 3 DP - 1995 Mar TI - Activation time determination by high-resolution unipolar and bipolar extracellular electrograms in the canine heart. PG - 174-88 AB - INTRODUCTION: To identify the optimal criteria for activation time (AT) determination of bipolar electrograms from normal hearts, a high-resolution cross electrode array comprising 128 unipolar electrodes of 500-microns spacing was used to record extracellular potentials from the left ventricular epicardium of 12 dog hearts. METHODS AND RESULTS: Recordings were made during broad wavefront propagation (B wave) and local elliptical wavefront propagation (E wave). Characteristics of 863 bipolar electrograms (1-mm spacing) were constructed from unipolar data standardized for differences in polarity, then classified morphologically. Features for bipolar AT determination were compared to the time of the negative peak of the first temporal derivative of a unipolar electrogram situated mid-way between the bipoles. During B wave, three distinct morphologies were observed: uniphasic (61%), biphasic (23%), and triphasic (16%). Peak voltage of uniphasic and triphasic signals was the best predictor of AT (error: 0.6 +/- 0.6 msec and 0.6 +/- 0.8 msec, respectively). During E wave, parallel orientation of the bipoles with respect to the direction of impulse propagation wavefront resulted in uniphasic signals (> 99%), while for perpendicular orientation of the bipoles, electrogram morphology was variable. For parallel orientation of the bipoles, peak negative voltage was the best predictor of AT for both longitudinal and transverse propagation, while for perpendicular bipole orientation, peak negative voltage was a less reliable predictor for propagation along both fiber axes. Increasing interpolar distance resulted in a degradation in AT accuracy for B wave (from 0.6 +/- 0.6 msec at 1 mm to 1.1 +/- 1.2 msec at 7 mm) and for E wave (from 0.4 +/- 0.3 msec at 1 mm to 3.1 +/- 2.9 msec at 7 mm). CONCLUSIONS: (1) The accuracy of bipolar electrograms is sensitive to wavefront direction, bipole orientation, and interpolar distance; (2) peak negative voltage of uniphasic and triphasic signals is a reliable predictor of AT, but only for B wave; (3) a maximum interpolar distance of 2 mm and bipole orientation parallel to the direction of the impulse wavefront are minimally required for accurate determination of AT during impulse propagation initiated near the recording electrodes; and (4) for impulses initiated near the recording site in normal tissue, a biphasic or triphasic morphology almost certainly indicates that the bipolar electrode is oriented perpendicular to the wavefront direction, irrespective of fiber orientation. FAU - Ndrepepa, G AU - Ndrepepa G AD - Department of Medicine, State University of New York Health Science Center, Brooklyn, USA. FAU - Caref, E B AU - Caref EB FAU - Yin, H AU - Yin H FAU - el-Sherif, N AU - el-Sherif N FAU - Restivo, M AU - Restivo M LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - J Cardiovasc Electrophysiol JT - Journal of cardiovascular electrophysiology JID - 9010756 SB - IM MH - Action Potentials/*physiology MH - Animals MH - Dogs MH - Electrocardiography/*classification MH - Electrodes MH - Heart/*physiology MH - Heart Conduction System/physiology MH - Heart Rate/*physiology MH - Male MH - Time Factors EDAT- 1995/03/01 00:00 MHDA- 1995/03/01 00:01 CRDT- 1995/03/01 00:00 PHST- 1995/03/01 00:00 [pubmed] PHST- 1995/03/01 00:01 [medline] PHST- 1995/03/01 00:00 [entrez] AID - 10.1111/j.1540-8167.1995.tb00769.x [doi] PST - ppublish SO - J Cardiovasc Electrophysiol. 1995 Mar;6(3):174-88. doi: 10.1111/j.1540-8167.1995.tb00769.x.