PMID- 7621404
OWN - NLM
STAT- MEDLINE
DCOM- 19950831
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 81
IP  - 2
DP  - 1995 Jun
TI  - DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have 
      totally different chromosome changes but the same genetic origin.
PG  - 103-8
AB  - Aneuploid DLD-1 and HCT-15 cell lines independently derived from a colon 
      carcinoma by two researchers lacked a common marker chromosome or a concurrent 
      numerical change. To further look into the genetic identity of these two cell 
      lines, we used chromosome painting with fluorescence in situ hybridization (FISH) 
      and confirmed the G-band identification on all chromosome changes. We have also 
      found comparable results between these two cell lines on C-/Q- polymorphic bands 
      of several chromosomes, and electrophoretic patterns of restriction 
      fragment-length polymorphisms (RFLPs) from eight Y-chromosome-specific DNA probes 
      with 13 restriction enzymes. These results strongly suggest that DLD-1 and HCT-15 
      are identical genetically. Clearly, cell lines bearing totally unrelated changes 
      in karotypes can be generated from the same cancer specimen.
FAU - Chen, T R
AU  - Chen TR
AD  - American Type Culture Collection, Rockville, MD 20852, USA.
FAU - Dorotinsky, C S
AU  - Dorotinsky CS
FAU - McGuire, L J
AU  - McGuire LJ
FAU - Macy, M L
AU  - Macy ML
FAU - Hay, R J
AU  - Hay RJ
LA  - eng
GR  - N01-CB-71014/CB/NCI NIH HHS/United States
GR  - N01-RR-92105/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Blotting, Southern
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/genetics
MH  - Chromosomes, Human/*genetics
MH  - Colorectal Neoplasms/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - Karyotyping
MH  - Polymorphism, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 0165-4608(94)00225-Z [pii]
AID - 10.1016/0165-4608(94)00225-z [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Jun;81(2):103-8. doi: 10.1016/0165-4608(94)00225-z.