PMID- 7624154
OWN - NLM
STAT- MEDLINE
DCOM- 19950831
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 11
IP  - 2
DP  - 1995 Jul 20
TI  - The Ras suppressor RSU-1 localizes to 10p13 and its expression in the U251 
      glioblastoma cell line correlates with a decrease in growth rate and tumorigenic 
      potential.
PG  - 397-403
AB  - Rsu-1, which was isolated based on its ability to suppress transformation by 
      v-Ras, is a highly conserved gene which shares homology with yeast adenylyl 
      cyclase in the region required for activation by Ras. Genomic DNA clones of human 
      RSU-1 have been isolated and used as a probe for fluorescence in situ 
      hybridization (FISH) to assign RSU-1 to 10p13, confirming the previous results of 
      somatic cell hybrid mapping localizing RSU-1 to chromosome 10. Screening of more 
      than 20 human tumor cell lines for RSU-1 expression revealed that most cell lines 
      contained abundant RSU-1 RNA and protein. However, the p33 RSU-1 protein was 
      undetectable in the U251 glioblastoma cell line and transfection of a rsu-1 
      expression vector into U251 cells yielded a cell line in which rsu-1 was under 
      the control of a regulatable metallothionein promoter. Addition of Cd2+ to the 
      U251-Rsu-1 transfectant resulted in transcription of rsu-1 RNA and the 
      accumulation of p33 Rsu-1 protein. Appearance of the Rsu-1 protein correlated 
      with a reduction in growth rate of the U251-Rsu-1 transfectant. In addition, 
      reduction in anchorage independent growth and phenotypic alteration in U251-Rsu-1 
      transfectant agar colonies was observed. Two U251-Rsu-1 transfectant cell lines 
      were non tumorigenic when injected subcutaneously into athymic nude mice. These 
      results, in conjunction with the frequent deletions observed in chromosome 10 in 
      glioblastomas, suggest that RSU-1 loss of function may play a role in the 
      progression of this disease.
FAU - Tsuda, T
AU  - Tsuda T
AD  - Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, 
      Maryland 20892-1750, USA.
FAU - Marinetti, M R
AU  - Marinetti MR
FAU - Masuelli, L
AU  - Masuelli L
FAU - Cutler, M L
AU  - Cutler ML
LA  - eng
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Complementary)
RN  - 00BH33GNGH (Cadmium)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
GS  - ras
GS  - rsp-1
GS  - rsu-1
MH  - Animals
MH  - Cadmium/pharmacology
MH  - Cell Division/*genetics
MH  - Cell Transformation, Neoplastic
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Gene Expression
MH  - *Genes, Tumor Suppressor/drug effects
MH  - *Genes, ras
MH  - Glioblastoma/*genetics/*pathology
MH  - Humans
MH  - In Situ Hybridization
MH  - Metallothionein/genetics
MH  - Mice
MH  - Mice, Nude
MH  - Phenotype
MH  - Promoter Regions, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1995/07/20 00:00
MHDA- 1995/07/20 00:01
CRDT- 1995/07/20 00:00
PHST- 1995/07/20 00:00 [pubmed]
PHST- 1995/07/20 00:01 [medline]
PHST- 1995/07/20 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1995 Jul 20;11(2):397-403.