PMID- 7624890
OWN - NLM
STAT- MEDLINE
DCOM- 19950829
LR  - 20190819
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 78
IP  - 3
DP  - 1995 Aug
TI  - Mineral fiber-induced leukocyte activation: the role of intra- and extracellular 
      calcium.
PG  - 195-205
AB  - The role of intra- and extracellular calcium in the activation of human 
      polymorphonuclear leukocytes (PMNL) to produce reactive oxygen metabolites (ROM) 
      were studied by using soluble, formyl-methionyl-leucyl-phenylalanine (fMLP) or 
      phorbol myristate acetate (PMA), or particulate stimuli, quartz or chrysotile. A 
      calcium channel inhibitor, verapamil, attenuated only quartz-induced elevation of 
      free intracellular calcium ([Ca2+]i) and ROM production. Likewise, 
      ethyleneglycol-bis (aminoethyl ether) tetraacetic acid (EGTA) attenuated quartz-, 
      chrysotile- and fMLP-induced elevation of [Ca2+]i and ROM production. It also 
      inhibited PMA-induced ROM production. A calcium ionophore, A23187 amplified ROM 
      production by all of these stimuli. These results suggest that both intra- and 
      extra-cellular calcium are required for the full activation of respiratory burst 
      by soluble and particulate stimuli in human PMNL.
FAU - Ruotsalainen, M
AU  - Ruotsalainen M
AD  - National Public Health Institute, Department of Toxicology, Kuopio, Finland.
FAU - Naarala, J
AU  - Naarala J
FAU - Savolainen, K M
AU  - Savolainen KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Asbestos, Serpentine)
RN  - 0 (Reactive Oxygen Species)
RN  - 14808-60-7 (Quartz)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - CJ0O37KU29 (Verapamil)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Asbestos, Serpentine/*toxicity
MH  - Calcimycin/pharmacology
MH  - Calcium/*metabolism
MH  - Cell Survival
MH  - Dose-Response Relationship, Drug
MH  - Egtazic Acid/pharmacology
MH  - Fura-2/chemistry
MH  - Humans
MH  - Luminescent Measurements
MH  - Lymphocyte Activation/*drug effects
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/metabolism
MH  - Quartz/*toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Respiratory Burst/drug effects
MH  - Spectrometry, Fluorescence
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Verapamil/pharmacology
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 037842749503255J [pii]
AID - 10.1016/0378-4274(95)03255-j [doi]
PST - ppublish
SO  - Toxicol Lett. 1995 Aug;78(3):195-205. doi: 10.1016/0378-4274(95)03255-j.