PMID- 7625073
OWN - NLM
STAT- MEDLINE
DCOM- 19950830
LR  - 20190819
IS  - 0042-9007 (Print)
IS  - 0042-9007 (Linking)
VI  - 68
IP  - 3
DP  - 1995
TI  - Establishment of a T-cell line from lymphocytes presumably implicated in 
      posttransfusion graft-versus-host disease.
PG  - 164-8
AB  - Posttransfusion graft-versus-host disease (PTGVHD) is known to develop in 
      immunocompetent patients exhibiting clinical symptoms such as erythroderma, 
      fever, liver dysfunction, diarrhea and pancytopenia. It is speculated that 
      transfused blood donors' lymphocytes might recognize the recipients' HLAs as 
      alloantigens. The thus stimulated lymphocytes might proliferate, expand and 
      finally attack the host's immune system or tissues. However, details regarding 
      these expanded donor cells such as: (1) whether they represent one clone or more, 
      (2) the composition of lymphocyte subsets, and (3) the target HLA antigens of 
      recipients, are not clear, since T-cell lines derived from PTGVHD patients have 
      not yet been obtained. The aim of this study is to characterize T-cells 
      responsible for PTGVHD and to identify their target molecules. For that purpose, 
      we attempted to establish T-cell lines derived from a PTGVHD patient. We show 
      that the established T-cell line, proven to be derived from donor lymphocytes, 
      showed a CD4+ phenotype and had cytotoxic activities. Furthermore, we describe 
      that the target of the cytotoxic T-cell line (CTL) is an HLA-DRB1*0405-related 
      molecule of the patient.
FAU - Nishimura, M
AU  - Nishimura M
AD  - Department of Research, Japanese Red Cross, Central Blood Center, Tokyo.
FAU - Uchida, S
AU  - Uchida S
FAU - Mitsunaga, S
AU  - Mitsunaga S
FAU - Tokunaga, K
AU  - Tokunaga K
FAU - Mitomi, Y
AU  - Mitomi Y
FAU - Tadokoro, K
AU  - Tadokoro K
FAU - Juji, T
AU  - Juji T
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Vox Sang
JT  - Vox sanguinis
JID - 0413606
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - Cytotoxicity, Immunologic
MH  - Female
MH  - Graft vs Host Disease/etiology/*pathology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocytes/*immunology
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - T-Lymphocytes/*pathology
MH  - *Transfusion Reaction
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1111/j.1423-0410.1995.tb03919.x [doi]
PST - ppublish
SO  - Vox Sang. 1995;68(3):164-8. doi: 10.1111/j.1423-0410.1995.tb03919.x.