PMID- 7626081
OWN - NLM
STAT- MEDLINE
DCOM- 19950831
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 212
IP  - 2
DP  - 1995 Jul 17
TI  - Redistribution of protein kinase C isoforms in human neutrophils stimulated by 
      formyl peptides and phorbol myristate acetate.
PG  - 664-72
AB  - The redistribution of protein kinase C (PKC) isoforms between the cytosolic and 
      plasma membrane fractions of stimulated human polymorphonuclear leukocytes (PMN) 
      was analysed by means of western blotting with antibodies against PKC beta I, 
      beta II and Zeta. Treatment of PMN with 1 microM 
      formyl-methionyl-leucyl-phenylalanine (fMLP) induced a rapid (5-10 sec) and 
      sustained (at least 10 min) increase in the membrane association of PKC beta I, 
      beta II, and the two immunoreactive proteins (76-81 kDa) recognized by the 
      antibody directed against PKC zeta. Optimal translocation of PKC isoforms to the 
      plasma membrane occurred in the presence of 10(-6) M fMLP and was not associated 
      with a detectable fall in cytosolic PKC. In the absence of external calcium, the 
      translocation of all PKC isoforms induced by fMLP was rapid (5 sec) but the 
      membrane association of PKC was lost within one minute. Unlike fMLP, phorbol 
      myristate acetate (PMA) induced a concentration-dependent translocation of the 
      PKC isoforms, which persisted in the membrane in the absence of external calcium. 
      These data provide the first evidence of redistribution of PKC isoforms by a 
      chemoattractant. They further indicate that external calcium plays a crucial role 
      in the persistence of the membrane association of PKC beta I, beta II and zeta 
      induced by formyl peptides.
FAU - Dang, P M
AU  - Dang PM
AD  - Laboratoire d'Hematologie, INSERM U294, CHU Xavier Bichat, Paris, France.
FAU - Rais, S
AU  - Rais S
FAU - Hakim, J
AU  - Hakim J
FAU - Perianin, A
AU  - Perianin A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Isoenzymes)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Blotting, Western
MH  - Calcium/pharmacology
MH  - Cell Membrane/enzymology
MH  - Cytosol/enzymology
MH  - Humans
MH  - Isoenzymes/*analysis/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/enzymology/*ultrastructure
MH  - Protein Kinase C/*analysis/metabolism
MH  - Tetradecanoylphorbol Acetate/*pharmacology
EDAT- 1995/07/17 00:00
MHDA- 1995/07/17 00:01
CRDT- 1995/07/17 00:00
PHST- 1995/07/17 00:00 [pubmed]
PHST- 1995/07/17 00:01 [medline]
PHST- 1995/07/17 00:00 [entrez]
AID - S0006-291X(85)72020-7 [pii]
AID - 10.1006/bbrc.1995.2020 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Jul 17;212(2):664-72. doi: 
      10.1006/bbrc.1995.2020.