PMID- 7626082
OWN - NLM
STAT- MEDLINE
DCOM- 19950831
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 212
IP  - 2
DP  - 1995 Jul 17
TI  - The amino-terminal fragment of the adenylate cyclase activating polypeptide 
      (PACAP) receptor functions as a high affinity PACAP binding domain.
PG  - 673-80
AB  - The PACAP receptor represents a member of a novel subfamily of G-protein coupled 
      receptors with a common structurally conserved extracellular domain of about 150 
      amino acids. We have addressed the question whether this extracellular 
      amino-terminus of the PACAP type I receptor can solely function as a PACAP 
      binding domain. For that purpose a cDNA was constructed that encodes the 
      membrane-anchored amino-terminus of the rat PACAP receptor including the 
      decapeptide epitope EQKLISEEDL for immunodetection. COS-7 cells were transfected 
      with this cDNA and a comparable construct of the wild-type receptor. Binding 
      analysis showed that the amino-terminal fragment of the PACAP receptor bound 
      PACAP with high-affinity (Kd = 3.8 nM; Bmax = 12.8 pmol/mg protein). In 
      comparison to the full-length receptor (Kd = 0.2 nM; Bmax = 1.96 pmol/mg protein) 
      its affinity was reduced by a factor of about 20. The results suggest that the 
      amino-terminus of the PACAP receptor functions as the major binding site for its 
      ligand.
FAU - Cao, Y J
AU  - Cao YJ
AD  - Max-Planck-Institut fur Biophysik, Kennedyallee, Germany.
FAU - Gimpl, G
AU  - Gimpl G
FAU - Fahrenholz, F
AU  - Fahrenholz F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adcyap1 protein, rat)
RN  - 0 (DNA, Complementary)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I)
RN  - 0 (Receptors, Pituitary Hormone)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - DNA, Complementary
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Neuropeptides/*metabolism
MH  - Peptide Fragments/*chemistry/genetics/*metabolism
MH  - Pituitary Adenylate Cyclase-Activating Polypeptide
MH  - Protein Conformation
MH  - Rats
MH  - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
MH  - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
MH  - Receptors, Pituitary Hormone/*chemistry/genetics/*metabolism
MH  - Recombinant Proteins
MH  - Transfection
EDAT- 1995/07/17 00:00
MHDA- 1995/07/17 00:01
CRDT- 1995/07/17 00:00
PHST- 1995/07/17 00:00 [pubmed]
PHST- 1995/07/17 00:01 [medline]
PHST- 1995/07/17 00:00 [entrez]
AID - S0006291X85720219 [pii]
AID - 10.1006/bbrc.1995.2021 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Jul 17;212(2):673-80. doi: 
      10.1006/bbrc.1995.2021.