PMID- 7627085
OWN - NLM
STAT- MEDLINE
DCOM- 19950906
LR  - 20191031
IS  - 0964-1955 (Print)
IS  - 0964-1955 (Linking)
VI  - 31B
IP  - 1
DP  - 1995 Jan
TI  - Cytokinetic studies of oral cancer cells using bromodeoxyuridine labelling in 
      relation to factors influencing prognosis.
PG  - 32-6
AB  - Cytokinetics of 27 untreated oral squamous cell carcinomas were evaluated by in 
      vitro bromodeoxyuridine (BrdU) labelling. A mononuclear cell suspension was 
      prepared for the labelling using collagenase and DNase treatment. The labelled 
      cells were visualised by immunofluorescence staining with an anti-BrdU monoclonal 
      antibody. The labelling index (LI%) was calculated by determining the percentage 
      of BrdU labelled cells. The LIs of the carcinomas ranged from 3.8 to 19.2%. The 
      LIs of poorly-differentiated (HG3) tumours were 2-3-fold higher compared with 
      those of well-differentiated (HG1) and moderately-differentiated (HG2) 
      carcinomas. Results also showed statistically significant (P < 0.0005) increases 
      in LIs from nuclear grade 1 (NG1) to nuclear grade 3 (NG3) carcinomas. Higher LIs 
      were observed in stage III/IV (10.82 +/- 4.62; 10.36 +/- 4.90) than those in 
      stage I (6.87 +/- 2.09) and II (7.14 +/- 1.87) carcinomas. A significant (P < 
      0.0005) difference in LI values was found between the patients with positive and 
      negative lymph nodes. Good correlation (r = 0.77) was exhibited between the LI 
      values and mitotic counts (MC) of the specimens. These results on oral cancer 
      cell proliferation seem to have prognostic implications.
FAU - Mukhopadhyay, D
AU  - Mukhopadhyay D
AD  - Department of Tumour Virology, Chittaranjan National Cancer Institute, Calcutta, 
      India.
FAU - Chatterjee, R
AU  - Chatterjee R
FAU - Chakraborty, R N
AU  - Chakraborty RN
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Cancer B Oral Oncol
JT  - European journal of cancer. Part B, Oral oncology
JID - 9214373
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bromodeoxyuridine
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Cell Cycle
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Mitotic Index
MH  - Mouth Neoplasms/*pathology
MH  - Prognosis
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1016/0964-1955(94)00041-2 [doi]
PST - ppublish
SO  - Eur J Cancer B Oral Oncol. 1995 Jan;31B(1):32-6. doi: 
      10.1016/0964-1955(94)00041-2.