PMID- 7627928
OWN - NLM
STAT- MEDLINE
DCOM- 19950905
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 82
IP  - 1
DP  - 1995 Jul 1
TI  - Molecular cytogenetics of human papillomavirus-negative cervical carcinoma cell 
      lines.
PG  - 1-8
AB  - Cervical cancer is a malignancy closely associated with human papillomavirus 
      (HPV). However, some cervical carcinomas occur in the absence of the virus. Two 
      HPV-negative cervical carcinoma cell lines, C-33A and HT-3, were examined by 
      G-banding and fluorescence in situ hybridization (FISH) using several whole 
      chromosome and satellite probes. Combined conventional banding and FISH analysis 
      significantly enhanced identification of complex and cryptic chromosome 
      rearrangements. Common to both cell lines were translocations of chromosome 1, 
      involving a specific site on the short arm and partial or complete loss of the 
      short arm of chromosome 9, as well as loss of chromosome 13. The C-33A line had a 
      relatively simple chromosomal constitution, with chromosome 1 being involved in 
      translocations with chromosomes 9, 18, and 21. Karyotype reconstruction of 
      G-banded and painted chromosomes indicate a net loss of DNA as a result of 
      unbalanced translocations, which occurred only at site 9p24 and loss of one copy 
      of chromosomes 13 and 14. In contrast, HT-3 cells had complex rearrangements and 
      deletions of chromosomes 1p, 3p, 9p, 10q/p, 11p/q, and 17p, all regions with 
      known tumor suppressor genes. The deletions observed in these HPV-negative 
      cervical carcinomas will be important in delineating regions of tumor suppressor 
      genes.
FAU - Zimonjic, D B
AU  - Zimonjic DB
AD  - Laboratory of Biology, National Cancer Institute, National Institutes of Health, 
      Bethesda, Maryland 20892, USA.
FAU - Simpson, S
AU  - Simpson S
FAU - Popescu, N C
AU  - Popescu NC
FAU - DiPaolo, J A
AU  - DiPaolo JA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Carcinoma/*pathology/virology
MH  - Chromosome Aberrations/*pathology
MH  - Chromosome Disorders
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Papillomaviridae
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/*pathology/virology
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 0165-4608(95)91129-7 [pii]
AID - 10.1016/0165-4608(95)91129-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Jul 1;82(1):1-8. doi: 10.1016/0165-4608(95)91129-7.