PMID- 7630005
OWN - NLM
STAT- MEDLINE
DCOM- 19950905
LR  - 20110727
IS  - 0047-1852 (Print)
IS  - 0047-1852 (Linking)
VI  - 53
IP  - 7
DP  - 1995 Jul
TI  - [Possible significance of VLA-4 (alpha 4 beta 1) for hematogenous metastasis of 
      renal cell cancer].
PG  - 1666-71
AB  - Very late antigen-4 (VLA-4) composed of alpha 4 and beta 1, a member of the beta 
      1-integrin subfamily, facilitates cell-to-cell interaction with vascular cell 
      adhesion molecule-1 (VCAM-1) on endothelial cells (EC). Attachment of blood-borne 
      tumor cells to EC is a crucial step for hematogenous metastasis, and 
      VLA-4-positive tumor cells can attach to EC by binding to VCAM-1. Renal cell 
      cancer (RCC) reveals proportionally greater percentage of metastasis among other 
      carcinomas at an initial diagnosis. We investigated whether VLA-4 is expressed on 
      RCC and how such expression on RCC correlates with metastatic potential of RCC. 
      Immunohistochemical staining on 66 primary and 4 metastatic RCCs showed that 4 of 
      4 metastatic and 5 of 8 primary RCCs (72.5%) from patients with lung and/or brain 
      metastasis expressed alpha 4 and beta 1 chains. On the other hand, 13 of 58 
      (22.4%) RCCs without metastasis expressed alpha 4 chain. alpha 4 and beta 1 
      expressions were also detected on 5 of 5 human RCC cell lines by flowcytometer 
      analysis. RT-PCR followed by Southern blot hybridization also confirmed mRNA 
      production in 4 of 5 RCC cell lines. Furthermore, adhesion of alpha 4-positive 
      RCC cell lines to human umbilical vein endothelial cells (HUVECs) was augmented 
      by a treatment of HUVECs with Tumor necrosis factor-alpha (TNF-alpha) or IL-4 
      thorough increase of VCAM-1 expression. These adhesion were inhibited by 
      anti-alpha 4 or anti-VCAM-1 antibodies suggesting that VLA-4-VCAM-1 interaction 
      was involved in the adhesion between RCC cells and HUVECs.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Tomita, Y
AU  - Tomita Y
AD  - Department of Urology, Niigata University School of Medicine.
FAU - Saito, K
AU  - Saito K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Nihon Rinsho
JT  - Nihon rinsho. Japanese journal of clinical medicine
JID - 0420546
RN  - 0 (Receptors, Very Late Antigen)
SB  - IM
MH  - Humans
MH  - Kidney Neoplasms/*pathology
MH  - *Neoplastic Cells, Circulating
MH  - Receptors, Very Late Antigen/*physiology
MH  - Tumor Cells, Cultured
RF  - 19
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Rinsho. 1995 Jul;53(7):1666-71.