PMID- 7630189
OWN - NLM
STAT- MEDLINE
DCOM- 19950905
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 9
IP  - 7
DP  - 1995 Jul
TI  - Fluorescence in situ hybridization analysis of minute marker chromosomes in 
      leukemia with monosomy 7.
PG  - 1154-8
AB  - Monosomy 7 was detected in bone marrow cells from three patients, one with 
      myeloid leukemia, and two others with myelodysplastic syndrome following previous 
      chemotherapy. Fluorescence in situ hybridization (FISH), carried out with an 
      alphoid DNA probe specific for chromosome 7 centromere, showed that a small 
      marker chromosome present in the tumor cells' karyotype of the three patients, 
      was derived from the missing chromosome 7. In two cases, the marker was a ring 
      chromosome, whereas in the third case it was a tiny dot-like chromosome, 
      unnoticed at first examination on R-banded metaphases. In the three cases, the 
      marker was lost in a proportion of tumor cells. FISH experiments suggested that 
      the marker centromere had undergone structural alterations, with a fluorescence 
      pattern distinct from a normal one. On the whole, these data suggest that: 
      firstly, leukemia-associated monosomy 7 results, in a proportion of cases, from a 
      structural event rather than from simple loss of a whole chromosome 7; secondly, 
      interpretation of interphase FISH must be cautious in monosomy 7 evaluation; and 
      thirdly structural alteration of the chromosome 7 derivative alphoid DNA could 
      explain its propensity to segregate unequally and to be lost at mitosis.
FAU - Viguie, F
AU  - Viguie F
AD  - Laboratoire de Biologie de la Fertilite et de Cytogenetique, Hopital Hotel-Dieu, 
      Paris, France.
FAU - Prigent, Y
AU  - Prigent Y
FAU - Ramond, S
AU  - Ramond S
FAU - Baumelou, E
AU  - Baumelou E
FAU - Cadiou, M
AU  - Cadiou M
FAU - Dreyfus, F
AU  - Dreyfus F
FAU - Zittoun, R
AU  - Zittoun R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosomes, Human, Pair 7
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Monocytic, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Monosomy
MH  - Myelodysplastic Syndromes/genetics
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1995 Jul;9(7):1154-8.