PMID- 7632924
OWN - NLM
STAT- MEDLINE
DCOM- 19950912
LR  - 20231213
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 28
IP  - 3
DP  - 1995 Jun
TI  - Extreme heterogeneity of polyadenylation sites in mRNAs encoding chloroplast 
      RNA-binding proteins in Nicotiana plumbaginifolia.
PG  - 569-74
AB  - We have previously characterized nuclear cDNA clones encoding two RNA binding 
      proteins, CP-RBP30 and CP-RBP-31, which are targeted to chloroplasts in Nicotiana 
      plumbaginifolia. In this report we describe the analysis of the 3'-untranslated 
      regions (3'-UTRs) in 22 CP-RBP30 and 8 CP-RBP31 clones which reveals that mRNAs 
      encoding both proteins have a very complex polyadenylation pattern. Fourteen 
      distinct poly(A) sites were identified among CP-RBP30 clones and four sites among 
      the CP-RBP31 clones. The authenticity of the sites was confirmed by RNase A/T1 
      mapping of N. plumbaginifolia RNA. CP-RBP30 provides an extreme example of the 
      heterogeneity known to be a feature of mRNA polyadenylation in higher plants. 
      Using PCR we have demonstrated that CP-RBP genes in N. plumbaginifolia and N. 
      sylvestris, in addition to the previously described introns interrupting the 
      coding region, contain an intron located in the 3' non-coding part of the gene. 
      In the case of the CP-RBP31, we have identified one polyadenylation event 
      occurring in this intron.
FAU - Klahre, U
AU  - Klahre U
AD  - Friedrich Miescher-Institut, Basel, Switzerland.
FAU - Hemmings-Mieszczak, M
AU  - Hemmings-Mieszczak M
FAU - Filipowicz, W
AU  - Filipowicz W
LA  - eng
SI  - GENBANK/X65117
SI  - GENBANK/X65118
PT  - Journal Article
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA-Binding Proteins)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - Base Sequence
MH  - Chloroplasts/*metabolism
MH  - DNA, Complementary
MH  - Hydrolysis
MH  - Molecular Sequence Data
MH  - *Plants, Toxic
MH  - Poly A/*metabolism
MH  - RNA, Messenger/genetics/*metabolism
MH  - RNA, Plant/genetics/metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Nicotiana/genetics/*metabolism
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1007/BF00020402 [doi]
PST - ppublish
SO  - Plant Mol Biol. 1995 Jun;28(3):569-74. doi: 10.1007/BF00020402.