PMID- 7632947
OWN - NLM
STAT- MEDLINE
DCOM- 19950914
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 86
IP  - 4
DP  - 1995 Aug 15
TI  - Delineation of an immunodominant and human T-cell lymphotropic virus 
      (HTLV)-specific epitope within the HTLV-I transmembrane glycoprotein.
PG  - 1392-9
AB  - Antibody reactivity to the transmembrane region of human T-cell lymphotropic 
      virus type I (HTLV-I) envelope, gp21, is observed in virtually all individuals 
      infected with HTLV-I or HTLV-II. Recombinant proteins encoding selected portions 
      of gp21 are described and used to define two immunogenic regions. The first 
      epitope (designated GD21-I) contains amino acids 361 to 404 of the HTLV-I 
      envelope and reacted with all of 54 sera from HTLV-I- and HTLV-II-infected 
      individuals. The second epitope (designated BA21) expresses amino acids 397 to 
      430 of the HTLV-I envelope and was recognized by 33 of 54 HTLV antisera. To 
      determine the specificity of GD21-I and BA21, sera from 17 HTLV-negative 
      individuals with nonspecific reactivity to p21E were tested. None of these sera 
      reacted with GD21-I, but 16 of 17 sera reacted with BA21. With virtually complete 
      reactivity to sera from HTLV-infected individuals and no reactivity to sera from 
      p21E-reactive uninfected individuals, GD21-I will be useful in immunoassays for 
      the detection of HTLV infection.
FAU - Hadlock, K G
AU  - Hadlock KG
AD  - Department of Molecular Virology, Genelabs Technologies, Inc. Redwood City, CA 
      94063, USA.
FAU - Goh, C J
AU  - Goh CJ
FAU - Bradshaw, P A
AU  - Bradshaw PA
FAU - Perkins, S
AU  - Perkins S
FAU - Lo, J
AU  - Lo J
FAU - Kaplan, J E
AU  - Kaplan JE
FAU - Khabbaz, R
AU  - Khabbaz R
FAU - Foung, S K
AU  - Foung SK
LA  - eng
GR  - DA60596/DA/NIDA NIH HHS/United States
GR  - HL33811/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (Gene Products, env)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
RN  - 0 (gp21 protein, Human T-lymphotropic virus 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Viral/*chemistry
MH  - Base Sequence
MH  - DNA Primers/chemistry
MH  - Epitope Mapping
MH  - Gene Products, env/*immunology
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/immunology
MH  - Retroviridae Proteins, Oncogenic/*immunology
MH  - Solubility
MH  - env Gene Products, Human Immunodeficiency Virus
EDAT- 1995/08/15 00:00
MHDA- 1995/08/15 00:01
CRDT- 1995/08/15 00:00
PHST- 1995/08/15 00:00 [pubmed]
PHST- 1995/08/15 00:01 [medline]
PHST- 1995/08/15 00:00 [entrez]
AID - S0006-4971(20)76396-2 [pii]
PST - ppublish
SO  - Blood. 1995 Aug 15;86(4):1392-9.