PMID- 7632954
OWN - NLM
STAT- MEDLINE
DCOM- 19950914
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 86
IP  - 4
DP  - 1995 Aug 15
TI  - Numerical chromosome aberrations are present within the CD30+ Hodgkin and 
      Reed-Sternberg cells in 100% of analyzed cases of Hodgkin's disease.
PG  - 1464-8
AB  - In Hodgkin's disease, cytogenetically aberrant clones have been demonstrated in a 
      minority of cases studied. In the remaining cases, only normal metaphases have 
      been found, but it is questionable whether normal karyotypes actually correspond 
      to the pathognomonic Hodgkin and Reed-Sternberg (HRS) cells. Numerical 
      aberrations could be studied by fluorescence in situ hybridization (FISH). 
      However, in Hodgkin's disease, the percentage of tumor cells is mostly below the 
      detection limit of FISH, which is near 1%. With the technique of simultaneous 
      fluorescence immunophenotyping and interphase cytogenetic analysis (FICTION), 
      this problem can be overcome. By FICTION, hybridization signals can selectively 
      be evaluated within the CD30a+ cell population. We have studied 30 
      cytogenetically analyzed cases of Hodgkin's disease by means of FICTION. In all 
      cases, we found numerical chromosome aberrations within the majority of CD30+ HRS 
      cells. In cases with complex and hyperdiploid karyotypes, the cytogenetic results 
      agreed with the FICTION data. There was considerable variability in the 
      chromosome numbers, demonstrating that karyotype instability is an in vivo 
      phenomenon of HRS cells. Lymphocytes never displayed numerical chromosome 
      changes. Our results indicate that HRS cells regularly exhibit numerical 
      chromosome aberrations and that the chromosome numbers are always in the 
      hyperploid range.
FAU - Weber-Matthiesen, K
AU  - Weber-Matthiesen K
AD  - Department of Human Genetics, University of Kiel, Germany.
FAU - Deerberg, J
AU  - Deerberg J
FAU - Poetsch, M
AU  - Poetsch M
FAU - Grote, W
AU  - Grote W
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Ki-1 Antigen)
SB  - IM
CIN - Blood. 1996 May 15;87(10):4479-80. PMID: 8639811
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Centromere
MH  - Chromosome Aberrations/*pathology
MH  - Chromosome Disorders
MH  - Female
MH  - Hodgkin Disease/immunology/*pathology
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Ki-1 Antigen/analysis
MH  - Male
MH  - Middle Aged
MH  - Reed-Sternberg Cells/*pathology
EDAT- 1995/08/15 00:00
MHDA- 1995/08/15 00:01
CRDT- 1995/08/15 00:00
PHST- 1995/08/15 00:00 [pubmed]
PHST- 1995/08/15 00:01 [medline]
PHST- 1995/08/15 00:00 [entrez]
AID - S0006-4971(20)76405-0 [pii]
PST - ppublish
SO  - Blood. 1995 Aug 15;86(4):1464-8.