PMID- 7632955
OWN - NLM
STAT- MEDLINE
DCOM- 19950914
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 86
IP  - 4
DP  - 1995 Aug 15
TI  - Translocations and amplification of the BCL2 gene are detected in interphase 
      nuclei of non-Hodgkin's lymphoma by in situ hybridization with yeast artificial 
      chromosome clones.
PG  - 1481-6
AB  - Translocation of the BCL2 gene in B-cell malignancies carrying t(14;18) and 
      amplification of the BCL2 gene in a cell line (HBL-2) derived from a 
      non-Hodgkin's lymphoma (NHL) were detected specifically in both metaphase spreads 
      and interphase nuclei by fluorescence in situ hybridization (FISH) using yeast 
      artificial chromosomes (YACs). A YAC clone containing the BCL2 gene yA153A6, a 
      360-kb clone spanning from approximately 60 kb upstream of BCL2 exon 1 to 
      approximately 60 kb 3' of the minor breakpoint cluster region, was used for 
      single-color FISH analysis. Seven patients with NHL and one patient with acute 
      lymphoblastic leukemia were analyzed for BCL2 translocations. Interphase nuclei 
      of NHL patients showed three signals when hybridized with the yA153A6 probe. This 
      was expected because the YAC clone spans the BCL2 breakpoint regions on 18q21.3. 
      In a patient with acute lymphoblastic leukemia, a positive signal for BCL2 was 
      detected on der(14) at band 14q32.33 by single-color FISH with the yA153A6 probe, 
      whereas no signals were detected on der(18). The amplification of BCL2 in the 
      HBL-2 cell line was observed on a characteristic abnormal chromosome 18, 
      add(18)(q23); the periodic pattern of the fluorescent signal of this region was 
      suggestive of an amplicon. Using double-color FISH with YAC clones containing the 
      more centromeric 18q21.3 gene gastrin-releasing peptide (y302F10) and the 
      14q32.33 gene (IgH; Y6), we detected t(14;18) by showing the juxtaposition of the 
      18q21.3 and 14q32.33 bands on the derivative chromosome 18. Interphase FISH with 
      these YAC clones provided a rapid procedure for the diagnosis of B-cell 
      malignancies carrying t(14;18). In addition, we showed that translocations and 
      amplification of the BCL2 gene can be detected at the single-cell level.
FAU - Taniwaki, M
AU  - Taniwaki M
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Sliverman, G A
AU  - Sliverman GA
FAU - Nishida, K
AU  - Nishida K
FAU - Horiike, S
AU  - Horiike S
FAU - Misawa, S
AU  - Misawa S
FAU - Shimazaki, C
AU  - Shimazaki C
FAU - Miura, I
AU  - Miura I
FAU - Nagai, M
AU  - Nagai M
FAU - Abe, M
AU  - Abe M
FAU - Fukuhara, S
AU  - Fukuhara S
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
GS  - BCL2
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*pathology
MH  - Chromosome Banding
MH  - Chromosome Disorders
MH  - Chromosomes, Artificial, Yeast
MH  - Female
MH  - Gene Amplification
MH  - Genes, Immunoglobulin
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphoma, Non-Hodgkin/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - Translocation, Genetic
EDAT- 1995/08/15 00:00
MHDA- 1995/08/15 00:01
CRDT- 1995/08/15 00:00
PHST- 1995/08/15 00:00 [pubmed]
PHST- 1995/08/15 00:01 [medline]
PHST- 1995/08/15 00:00 [entrez]
AID - S0006-4971(20)76407-4 [pii]
PST - ppublish
SO  - Blood. 1995 Aug 15;86(4):1481-6.