PMID- 7633400
OWN - NLM
STAT- MEDLINE
DCOM- 19950911
LR  - 20220316
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 4
IP  - 4
DP  - 1995 Apr
TI  - Ultra-sensitive FISH using peroxidase-mediated deposition of biotin- or 
      fluorochrome tyramides.
PG  - 529-34
AB  - We describe a detection principle for indirect fluorescence in situ hybridization 
      (FISH) methods that with only one or two antibody layers dramatically improves 
      FISH signal intensities. The method uses as a first layer an anti-hapten 
      immunoglobulin [or (strept)avidin] conjugated to peroxidase. The quintessence of 
      the method is the use of fluorochrome- or biotin labelled tyramides as peroxidase 
      substrates to generate and deposit many fluorochrome or biotin molecules close to 
      the in situ bound peroxidase. These may either be directly evaluated under the 
      fluorescence microscope or after another incubation with fluorochrome-labelled 
      (strept)avidin.
FAU - Raap, A K
AU  - Raap AK
AD  - Department of Cytochemistry and Cytometry, Medical Faculty, Leiden University, 
      The Netherlands.
FAU - van de Corput, M P
AU  - van de Corput MP
FAU - Vervenne, R A
AU  - Vervenne RA
FAU - van Gijlswijk, R P
AU  - van Gijlswijk RP
FAU - Tanke, H J
AU  - Tanke HJ
FAU - Wiegant, J
AU  - Wiegant J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Coumarins)
RN  - 0 (Fluoresceins)
RN  - 0 (Rhodamines)
RN  - 62669-72-1 (tetramethylrhodamine)
RN  - 6SO6U10H04 (Biotin)
RN  - EC 1.11.1.- (Peroxidases)
RN  - TPY09G7XIR (Fluorescein)
RN  - X8ZC7V0OX3 (Tyramine)
SB  - IM
MH  - Animals
MH  - Biotin/chemistry
MH  - Coumarins/chemistry
MH  - Fluorescein
MH  - Fluoresceins/chemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Peroxidases/*metabolism
MH  - Rhodamines/chemistry
MH  - Sensitivity and Specificity
MH  - Tyramine/*analogs & derivatives/metabolism
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1093/hmg/4.4.529 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1995 Apr;4(4):529-34. doi: 10.1093/hmg/4.4.529.