PMID- 7633442
OWN - NLM
STAT- MEDLINE
DCOM- 19950914
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 4
IP  - 5
DP  - 1995 May
TI  - High-resolution DNA Fiber-FISH for genomic DNA mapping and colour bar-coding of 
      large genes.
PG  - 831-6
AB  - We have applied two-colour fluorescence in situ hybridization (FISH) to DNA 
      fibers and combined it with digital imaging microscopy for the mapping of large 
      cosmid contigs. The technique was validated using a set of unique plasmids and a 
      cosmid contig both originating from the thyroglobulin (Tg) gene and previously 
      mapped by restriction analysis. The resolution proved to be close to the 
      theoretical lower limit of approximately 1 kb, ranging > or = 400 kb. 
      Subsequently a 400 kb cosmid contig derived from a DMD-YAC was directly mapped by 
      Fiber-FISH. The resulting map is in full agreement with the restriction map. 
      Two-colour Fiber-FISH mapping thus showed to be capable for accurately sizing 
      gaps and overlaps, and to identify chimeric or repeat sequence containing cosmids 
      across a 400 kb region at once. The generated 400 kb 'colour bar-code' was 
      subsequently used to map two DMD deletion breakpoints in patient DNA with an 
      accuracy of 1-2 kb. The results underscore the value of this method for the 
      delineation of chromosomal rearrangements for positional cloning and single 
      patient clinical studies.
FAU - Florijn, R J
AU  - Florijn RJ
AD  - Department of Cytochemistry and Cytometry, State University Leiden, The 
      Netherlands.
FAU - Bonden, L A
AU  - Bonden LA
FAU - Vrolijk, H
AU  - Vrolijk H
FAU - Wiegant, J
AU  - Wiegant J
FAU - Vaandrager, J W
AU  - Vaandrager JW
FAU - Baas, F
AU  - Baas F
FAU - den Dunnen, J T
AU  - den Dunnen JT
FAU - Tanke, H J
AU  - Tanke HJ
FAU - van Ommen, G J
AU  - van Ommen GJ
FAU - Raap, A K
AU  - Raap AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 9007-49-2 (DNA)
RN  - 9010-34-8 (Thyroglobulin)
SB  - IM
GS  - DMD
GS  - Tg
MH  - Chimera/genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Artificial, Yeast
MH  - Cloning, Molecular
MH  - Color
MH  - Cosmids/genetics
MH  - DNA/*genetics
MH  - Gene Deletion
MH  - Gene Rearrangement
MH  - Genome, Human
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Muscular Dystrophies/genetics
MH  - Plasmids/genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Thyroglobulin/genetics
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1093/hmg/4.5.831 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1995 May;4(5):831-6. doi: 10.1093/hmg/4.5.831.