PMID- 7635182
OWN - NLM
STAT- MEDLINE
DCOM- 19950914
LR  - 20061115
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 23
IP  - 9
DP  - 1995 Aug
TI  - Monocyte chemoattractant protein-1 stimulates tumor necrosis and recruitment of 
      macrophages into tumors in tumor-bearing nude mice: increased granulocyte and 
      macrophage progenitors in murine bone marrow.
PG  - 1035-9
AB  - Monocyte chemoattractant protein-1 (MCP-1) belongs to the newly recognized 
      "chemokine" superfamily of activation-inducible cytokines. We report here that 
      MCP-1 gene-transferred mouse myeloma cells modulate tumor necrosis in 
      myeloma-bearing nude mice. We established an MCP-1-producing myeloma cell line 
      (X63-MCP-1) by transfection with human MCP-1 cDNA as well as 
      interleukin-8-producing X63 cells (X63 IL-8). Each cell line showed the same 
      growth characteristics in vitro, and 1 x 10(7) cells per mouse were injected into 
      the peritoneal cavity resulting in the formation of tumors. Hematologic studies, 
      including peripheral white blood cell counts and differentiation, showed no 
      differences among the groups. They formed tumors in the same manner, which we 
      observed from weeks 2.5 to 9. MCP-1 mice showed more tumor necrosis and 
      infiltration of the macrophages into the tissue surrounding the tumor. In situ 
      hybridization, using a partial cDNA as a probe, showed that macrophages contained 
      MCP-1 mRNA. Bone marrow cell colony-forming assay showed a greater number of both 
      granulocyte and macrophage colonies in MCP-1 mouse femur than in those of 
      controls or interleukin-8 mice. MCP-1 has no direct stimulatory activity on stem 
      cells, but longer exposure to MCP-1 in vivo might stimulate both granulocyte and 
      macrophage progenitors and recruitment of macrophages into tumors, and it might 
      explain the antitumor activity of macrophages in tumor-bearing nude mice.
FAU - Hoshino, Y
AU  - Hoshino Y
AD  - Department of Internal Medicine, Jichi Medical School, Japan.
FAU - Hatake, K
AU  - Hatake K
FAU - Kasahara, T
AU  - Kasahara T
FAU - Takahashi, Y
AU  - Takahashi Y
FAU - Ikeda, M
AU  - Ikeda M
FAU - Tomizuka, H
AU  - Tomizuka H
FAU - Ohtsuki, T
AU  - Ohtsuki T
FAU - Uwai, M
AU  - Uwai M
FAU - Mukaida, N
AU  - Mukaida N
FAU - Matsushima, K
AU  - Matsushima K
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Ascites
MH  - Bone Marrow/*pathology
MH  - Cell Line
MH  - Chemokine CCL2
MH  - Chemotactic Factors/biosynthesis/*physiology
MH  - Cytokines/*physiology
MH  - Female
MH  - Granulocytes/*pathology
MH  - Hematopoietic Stem Cells/*pathology
MH  - In Situ Hybridization
MH  - Interleukin-8/biosynthesis/*physiology
MH  - Leukocyte Count
MH  - Macrophages/*pathology
MH  - Mice
MH  - Mice, Nude
MH  - Multiple Myeloma/blood/*pathology
MH  - Necrosis
MH  - Neoplasm Transplantation
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Recombinant Proteins/biosynthesis
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1995 Aug;23(9):1035-9.