PMID- 7635463
OWN - NLM
STAT- MEDLINE
DCOM- 19950911
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 96
IP  - 2
DP  - 1995 Aug
TI  - Isolation and chromosomal localization of a human ATP-regulated potassium 
      channel.
PG  - 155-60
AB  - KATP channels are K+ channels whose activity is inhibited by the presence of and 
      enhanced by the absence of cytosolic ATP. This property allows KATP channels to 
      sense cellular intermediary metabolism and directly couple this information to 
      the modulation of membrane excitability. Indeed, recent studies from our 
      laboratory and others have suggested that activation of KATP channels during 
      anoxia is important in the response and adaptation of central neurons to hypoxia. 
      In order to identify KATP channels from human brain, we performed a polymerase 
      chain reaction (PCR) using human cerebral cortex mRNA and primers derived from 
      the ROMK1 sequence, a cDNA clone encoding an ATP-regulated potassium channel, 
      recently isolated from rat kidney. We thus identified a novel 308-bp PCR product, 
      pKCNJ1, whose expression was found to be restricted to a 3.0-kb band in the 
      kidney by probing a human multiple tissue northern blot, pKCNJ1 was then used to 
      isolate genomic clones and, using fluorescence in situ hybridization (FISH) to 
      human metaphase chromosomes, was mapped to chromosome 11q.
FAU - Krishnan, S N
AU  - Krishnan SN
AD  - Department of Pediatrics (Section of Respiratory Medicine), Yale University 
      School of Medicine, New Haven, CT 06520-8064, USA.
FAU - Desai, T
AU  - Desai T
FAU - Ward, D C
AU  - Ward DC
FAU - Haddad, G G
AU  - Haddad GG
LA  - eng
SI  - GENBANK/S78737
GR  - HD15736/HD/NICHD NIH HHS/United States
GR  - HD28 940/HD/NICHD NIH HHS/United States
GR  - NS32578/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (KCNJ1 protein, human)
RN  - 0 (Kcnj1 protein, rat)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Inwardly Rectifying)
RN  - 0 (RNA, Messenger)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Ion Channel Gating
MH  - Kidney/chemistry
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Potassium Channels/drug effects/*genetics
MH  - *Potassium Channels, Inwardly Rectifying
MH  - RNA, Messenger/analysis
MH  - Sequence Homology, Amino Acid
MH  - Temporal Lobe/chemistry
MH  - Tissue Distribution
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1007/BF00207372 [doi]
PST - ppublish
SO  - Hum Genet. 1995 Aug;96(2):155-60. doi: 10.1007/BF00207372.