PMID- 7636622
OWN - NLM
STAT- MEDLINE
DCOM- 19950913
LR  - 20041117
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 176
IP  - 2
DP  - 1995 Jun
TI  - Numerical aberrations of chromosomes 1 and 7 in renal cell carcinomas as detected 
      by interphase cytogenetics.
PG  - 123-35
AB  - Alcohol-fixed single cell suspensions of 37 renal cell carcinomas (RCCs) were 
      assessed by both flow cytometry (FCM) and the fluorescence in situ hybridization 
      (FISH) technique, using chromosome 1- and chromosome 7-specific centromere DNA 
      probes. DNA diploidy or near-diploidy was observed in 30 of the 37 RCCs and only 
      12 of these (near-)diploid tumours were disomic for both chromosomes 1 and 7. 
      Numerical aberrations of chromosome 1 and/or chromosome 7 were present in 18 of 
      the 30 (near-)diploid RCCs and five of these cases showed monosomy for chromosome 
      1 in more than 50 per cent of the tumour cells. A double target FISH, with a 
      centromeric and a telomeric specific probe for 1p36, excluded misinterpretation 
      on the basis of clustering of 1q12, and suggested a complete loss of chromosome 
      1. All these five (near-)diploid RCCs with monosomy for chromosome 1 were 
      eosinophilic chromophilic cell carcinomas, according to the Thoenes 
      classification of RCC. This observation is of special interest, because it was 
      recently concluded from cytogenetic studies that the diagnosis of chromophilic 
      renal cell carcinoma must be considered as obsolete. Monosomy for chromosome 1 
      seems to be a non-random numerical aberration of (near-)diploid eosinophilic 
      chromophilic cell carcinomas, and a gain of one or more chromosomes 1 appeared to 
      be a common phenomenon in RCCs, especially in the DNA aneuploid tumours. As these 
      chromosomal abnormalities were not found in the earlier classical cytogenetic 
      studies, we conclude that in situ hybridization techniques are required in 
      addition to chromosome banding techniques to obtain a complete characterization 
      of the chromosome imbalances in RCCs.
FAU - Beck, J L
AU  - Beck JL
AD  - Department of Pathology, University Hospital Nijmegen, The Netherlands.
FAU - Hopman, A H
AU  - Hopman AH
FAU - Feitz, W F
AU  - Feitz WF
FAU - Schalken, J
AU  - Schalken J
FAU - Schaafsma, H E
AU  - Schaafsma HE
FAU - Van de Kaa, C A
AU  - Van de Kaa CA
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Hanselaar, A G
AU  - Hanselaar AG
FAU - De Wilde, P C
AU  - De Wilde PC
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - Carcinoma, Renal Cell/*genetics
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 7
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Kidney Neoplasms/*genetics
MH  - Monosomy
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1002/path.1711760205 [doi]
PST - ppublish
SO  - J Pathol. 1995 Jun;176(2):123-35. doi: 10.1002/path.1711760205.