PMID- 7636623
OWN - NLM
STAT- MEDLINE
DCOM- 19950913
LR  - 20111117
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 176
IP  - 2
DP  - 1995 Jun
TI  - Diagnosis of Ewing's sarcoma and related tumours by detection of chromosome 22q12 
      translocations using fluorescence in situ hybridization on tumour touch imprints.
PG  - 137-42
AB  - It is increasingly recognized that the identification of t(11;22)(q24;q12) is a 
      useful aid in the accurate diagnosis of Ewing's sarcoma and related tumours. 
      However, cytogenetic studies have a low success rate and adequate tumour is not 
      always available. This study describes the use of fluorescence in situ 
      hybridization (FISH) to detect translocations at 22q12, the site of the EWS gene 
      involved in t(11;22)(q24;q12), on tumour touch imprints made from true cut 
      core-needle biopsy and frozen tumour. Of the seven tumours analysed, five 
      diagnosed as Ewing's sarcoma or primitive neuroectodermal tumour demonstrated 
      chromosome translocation at 22q12. This is a rapid and reliable method to detect 
      a diagnostically relevant chromosome translocation using minimal amounts of fresh 
      or frozen tumour.
FAU - McManus, A P
AU  - McManus AP
AD  - Section of Paediatrics, Institute of Cancer Research, Sutton, Surrey, U.K.
FAU - Gusterson, B A
AU  - Gusterson BA
FAU - Pinkerton, C R
AU  - Pinkerton CR
FAU - Shipley, J M
AU  - Shipley JM
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - Adolescent
MH  - Bone Neoplasms/diagnosis/genetics
MH  - Cell Line
MH  - *Chromosomes, Human, Pair 22
MH  - Cosmids
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphocytes
MH  - Male
MH  - Neuroectodermal Tumors, Primitive/diagnosis
MH  - Sarcoma, Ewing/*diagnosis/genetics
MH  - Soft Tissue Neoplasms/diagnosis/genetics
MH  - *Translocation, Genetic
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1002/path.1711760206 [doi]
PST - ppublish
SO  - J Pathol. 1995 Jun;176(2):137-42. doi: 10.1002/path.1711760206.