PMID- 7638210
OWN - NLM
STAT- MEDLINE
DCOM- 19950911
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 16
DP  - 1995 Aug 1
TI  - Plaque-associated expression of human herpesvirus 6 in multiple sclerosis.
PG  - 7440-4
AB  - Representational difference analysis was used to search for pathogens in multiple 
      sclerosis brains. We detected a 341-nucleotide fragment that was 99.4% identical 
      to the major DNA binding protein gene of human herpesvirus 6 (HHV-6). Examination 
      of 86 brain specimens by PCR demonstrated that HHV-6 was present in > 70% of MS 
      cases and controls and is thus a commensal virus of the human brain. By DNA 
      sequencing, 36/37 viruses from MS cases and controls were typed as HHV-6 variant 
      B group 2. Other herpesviruses, retroviruses, and measles virus were detected 
      infrequently or not at all. HHV-6 expression was examined by immunocytochemistry 
      with monoclonal antibodies against HHV-6 virion protein 101K and DNA binding 
      protein p41. Nuclear staining of oligodendrocytes was observed in MS cases but 
      not in controls, and in MS cases it was observed around plaques more frequently 
      than in uninvolved white matter. MS cases showed prominent cytoplasmic staining 
      of neurons in gray matter adjacent to plaques, although neurons expressing HHV-6 
      were also found in certain controls. Since destruction of oligodendrocytes is a 
      hallmark of MS, these studies suggest an association of HHV-6 with the etiology 
      or pathogenesis of MS.
FAU - Challoner, P B
AU  - Challoner PB
AD  - PathoGenesis Corporation, Seattle, WA 98119, USA.
FAU - Smith, K T
AU  - Smith KT
FAU - Parker, J D
AU  - Parker JD
FAU - MacLeod, D L
AU  - MacLeod DL
FAU - Coulter, S N
AU  - Coulter SN
FAU - Rose, T M
AU  - Rose TM
FAU - Schultz, E R
AU  - Schultz ER
FAU - Bennett, J L
AU  - Bennett JL
FAU - Garber, R L
AU  - Garber RL
FAU - Chang, M
AU  - Chang M
AU  - et al.
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Antigens, Viral/metabolism
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics/isolation & purification
MH  - Herpesviridae Infections/complications/pathology/virology
MH  - Herpesvirus 6, Human/*genetics/*isolation & purification/pathogenicity
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Multiple Sclerosis/etiology/pathology/*virology
MH  - Oligodendroglia/virology
MH  - Polymerase Chain Reaction
PMC - PMC41355
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
PMCR- 1996/02/01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
PHST- 1996/02/01 00:00 [pmc-release]
AID - 10.1073/pnas.92.16.7440 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7440-4. doi: 10.1073/pnas.92.16.7440.