PMID- 7639639
OWN - NLM
STAT- MEDLINE
DCOM- 19950912
LR  - 20190717
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 40
IP  - 5
DP  - 1995 May
TI  - The role of brain acetylcholine in phenol-induced tremor in mice.
PG  - 365-72
AB  - The relation between phenol-induced tremor and brain acetylcholine levels, and 
      the effects of cholinergic drugs on the tremor were investigated, using male ddY 
      mice weighing 28-35 g. The magnitude of phenol-induced tremor was graded on a 
      4-point scale: 0, normal; 1, slight; 2, moderate; 3, severe, and acetylcholine 
      levels were measured by high-performance liquid chromatography with 
      electrochemical detection. The magnitude of tremor and the decrease in 
      acetylcholine levels in the striatum and cerebral cortex induced by s.c. 
      injection of phenol (50, 100, 200 mg/kg) were dose-dependent, and both showed 
      parallel time courses. Intracerebroventricular (i.c.v.) injection of phenol (100 
      micrograms/mouse) caused a decrease in acetylcholine levels in the striatum but 
      did not induce tremor. Intraperitoneal injection of pentobarbital (10 mg/kg), 
      which inhibits the release of acetylcholine, weakened both the magnitude of the 
      tremor and the decrease in acetylcholine levels induced by phenol (200 mg/kg), 
      whereas i.c.v. injection of pentobarbital (120 micrograms/mouse) attenuated the 
      decrease in acetylcholine levels induced by phenol, but did not affect the 
      magnitude of the tremor. Intraperitoneal (20 mg/kg) or i.c.v. (60 
      micrograms/mouse) injection of mecamylamine further strengthened the tremor. 
      Intraperitoneal (0.3 mg/kg) injection of physostigmine strengthened the tremor, 
      while i.c.v. injection (1 microgram/mouse) caused a reduction in its magnitude. 
      These results suggest that the tremor is caused directly by the phenol-induced 
      increase in acetylcholine release in the peripheral nervous system (motor nerve 
      endings), that the decrease in brain acetylcholine levels may be due to 
      phenol-induced increases in acetylcholine release within the central nervous 
      system, and that the resultant reduction in brain acetylcholine levels indirectly 
      suppresses the tremor.
FAU - Itoh, M
AU  - Itoh M
AD  - Department of Pharmacology, School of Dentistry, Iwate Medical University, 
      Morioka, Japan.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Phenols)
RN  - 6EE945D3OK (Mecamylamine)
RN  - 9U1VM840SP (Physostigmine)
RN  - I4744080IR (Pentobarbital)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/analysis/antagonists & inhibitors/*physiology
MH  - Analysis of Variance
MH  - Animals
MH  - Brain Chemistry/*drug effects
MH  - Cerebral Cortex/chemistry
MH  - Corpus Striatum/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Injections, Intraperitoneal
MH  - Injections, Intraventricular
MH  - Injections, Subcutaneous
MH  - Male
MH  - Mecamylamine/toxicity
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Parasympathetic Nervous System/*drug effects
MH  - Pentobarbital/pharmacology
MH  - Phenols/administration & dosage/*toxicity
MH  - Physostigmine/toxicity
MH  - Statistics, Nonparametric
MH  - Time Factors
MH  - Tremor/*chemically induced/physiopathology
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 0003-9969(94)00191-D [pii]
AID - 10.1016/0003-9969(94)00191-d [doi]
PST - ppublish
SO  - Arch Oral Biol. 1995 May;40(5):365-72. doi: 10.1016/0003-9969(94)00191-d.