PMID- 7641204
OWN - NLM
STAT- MEDLINE
DCOM- 19950918
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 55
IP  - 17
DP  - 1995 Sep 1
TI  - Multiple myeloma: high incidence of chromosomal aneuploidy as detected by 
      interphase fluorescence in situ hybridization.
PG  - 3854-9
AB  - Because metaphase cytogenetic studies in multiple myeloma (MM) are hampered by a 
      low proliferative activity of myeloma cells in vitro, interphase cytogenetics by 
      means of fluorescence in situ hybridization (FISH) should improve the detection 
      of chromosomal abnormalities in MM. We therefore investigated chromosomal 
      aneuploidy in 36 patients with MM using interphase FISH and alpha-satellite DNA 
      probes for chromosomes 1, 3, 7, 8, 11, 12, 16, 17, 18, and X. By FISH, myeloma 
      cells from 32 patients (88.9%) were aneuploid for at least one of the chromosomes 
      examined. In 24 patients (66%), aberrations of > or = 3 chromosomes were 
      observed. Aneuploidy was predominantly characterized by a gain of chromosome 
      numbers, with involvement of chromosomes 3, 7, and 11 occurring in > 50% of 
      patients. Loss of a centromeric signal suggesting monosomy was most frequently 
      observed for chromosomes 17 (22.2% of patients) and X (monosomic in 42.3% of 
      female patients, but loss of chromosome X was never observed in males, P < 0.05). 
      Dual-color FISH studies provided evidence for marked heterogeneity of aneuploid 
      cells in 8 patients (22.8%). Occurrence of chromosomal aneuploidy was independent 
      of stage and pretreatment status. Gain of chromosome 3 was significantly 
      correlated with an IgA paraprotein (P < 0.05). In 12 patients, the direct 
      comparison of metaphase cytogenetics and FISH showed that FISH detected 
      aneuploidy of chromosomes in 9 patients that was missed by metaphase analysis. In 
      conclusion, interphase FISH, by which chromosomal aneuploidy was detected in 
      almost 90% of patients with MM, represents an approach for evaluating the 
      clinical significance of specific chromosomal abnormalities in MM.
FAU - Drach, J
AU  - Drach J
AD  - First Department of Internal Medicine, University of Vienna, Austria.
FAU - Schuster, J
AU  - Schuster J
FAU - Nowotny, H
AU  - Nowotny H
FAU - Angerler, J
AU  - Angerler J
FAU - Rosenthal, F
AU  - Rosenthal F
FAU - Fiegl, M
AU  - Fiegl M
FAU - Rothermundt, C
AU  - Rothermundt C
FAU - Gsur, A
AU  - Gsur A
FAU - Jager, U
AU  - Jager U
FAU - Heinz, R
AU  - Heinz R
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aneuploidy
MH  - Cytogenetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1995 Sep 1;55(17):3854-9.