PMID- 7641424
OWN - NLM
STAT- MEDLINE
DCOM- 19950915
LR  - 20191023
IS  - 0262-0898 (Print)
IS  - 0262-0898 (Linking)
VI  - 13
IP  - 5
DP  - 1995 Sep
TI  - Inhibition of cancer cell motility and invasion by interleukin-12.
PG  - 396-404
AB  - Tumour cell motility and attachment are crucial requirements in the formation of 
      metastatic lesions. These properties are affected by a number of cytokines 
      including hepatocyte growth factor/scatter factor (HGF/SF) and several 
      immunoregulatory proteins, including interleukin-12 (IL-12). Although IL-12 has 
      been reported to exhibit potent anti-tumour effects in vivo, a direct effect of 
      IL-12 on cancer cells has not been reported. We show here that IL-12 directly 
      inhibited the attachment of the human colon cancer cell lines HRT18, HT29 and 
      HT115 to Matrigel, HGF/SF-stimulated cell motility and HGF/SF-induced cell 
      invasion through a reconstituted basement membrane. IL-12 did not affect the 
      growth of these cell lines. Flow cytometry, Western analysis and 
      immunohistochemistry revealed an up-regulation of E-cadherin cell-surface 
      adhesion molecules. These direct effects of IL-12 on colon cancer cells suggest a 
      potentially important role for IL-12 in metastasis.
FAU - Hiscox, S
AU  - Hiscox S
AD  - Department of Surgery, University of Wales College of Medicine, Cardiff, UK.
FAU - Hallett, M B
AU  - Hallett MB
FAU - Puntis, M C
AU  - Puntis MC
FAU - Jiang, W G
AU  - Jiang WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Exp Metastasis
JT  - Clinical & experimental metastasis
JID - 8409970
RN  - 0 (Cadherins)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Blotting, Western
MH  - Cadherins/analysis
MH  - Cell Adhesion/drug effects
MH  - Cell Death/drug effects
MH  - Cell Membrane/chemistry
MH  - Cell Movement/drug effects
MH  - Cell Transformation, Neoplastic
MH  - Colonic Neoplasms/drug therapy/*pathology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-12/*pharmacology
MH  - *Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - T-Lymphocytes, Cytotoxic/drug effects
MH  - Tumor Cells, Cultured
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1007/BF00121916 [doi]
PST - ppublish
SO  - Clin Exp Metastasis. 1995 Sep;13(5):396-404. doi: 10.1007/BF00121916.