PMID- 7641817
OWN - NLM
STAT- MEDLINE
DCOM- 19950918
LR  - 20151119
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 219
IP  - 2
DP  - 1995 Aug
TI  - HGF/SF inhibits junctional communication.
PG  - 657-63
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a fibroblast-derived protein 
      that affects the growth, motility, and differentiation of epithelial and 
      endothelial cells. We have investigated the effect of HGF/SF on junctional 
      communication in mouse keratinocytes (MK cells). HGF/SF inhibited cell 
      communication in MK cells as assessed by the transfer of a low-molecular-weight 
      dye, Lucifer Yellow. The inhibition was rapid, the earliest effects being 
      apparent 5 to 10 min after addition of the factor, and was transient. The 
      decrease in dye transfer correlated with a loss of the gap junction protein 
      connexin 43 as measured by Western blotting, probably due to increased protein 
      degradation. The results show that junctional communication is an early target of 
      HGF/SF activity and they are consistent with the hypothesis that gap junctions 
      are primary targets of the action of growth factors.
FAU - Moorby, C D
AU  - Moorby CD
AD  - Imperial Cancer Research Fund, Cambridge University Medical School, United 
      Kingdom.
FAU - Stoker, M
AU  - Stoker M
FAU - Gherardi, E
AU  - Gherardi E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Isoquinolines)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9654F8OVKE (lucifer yellow)
SB  - IM
MH  - Animals
MH  - Cell Communication/*drug effects
MH  - Cell Line
MH  - Gap Junctions/drug effects
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Isoquinolines
MH  - Keratinocytes/*cytology/drug effects
MH  - Mice
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - S0014-4827(85)71276-1 [pii]
AID - 10.1006/excr.1995.1276 [doi]
PST - ppublish
SO  - Exp Cell Res. 1995 Aug;219(2):657-63. doi: 10.1006/excr.1995.1276.