PMID- 7641915
OWN - NLM
STAT- MEDLINE
DCOM- 19950921
LR  - 20170920
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 64
IP  - 3
DP  - 1995 Sep
TI  - Ovarian 11 beta-hydroxysteroid dehydrogenase activity is inversely related to the 
      outcome of in vitro fertilization-embryo transfer treatment cycles.
PG  - 590-8
AB  - OBJECTIVES: To determine the relationship of ovarian 11 beta-hydroxysteroid 
      dehydrogenase (11 beta-HSD) activity to the outcome of IVF-ET and to establish 
      whether 11 beta-HSD activities vary for a given patient between consecutive 
      treatment cycles. DESIGN: Retrospective correlation analysis. SETTING: Patients 
      were treated and ovarian 11 beta-HSD activities were measured at independent 
      fertility and biochemistry departments, respectively. PATIENTS: Random series of 
      137 women undergoing a total of 172 treatment cycles for IVF-ET. INTERVENTIONS: 
      Subcutaneous buserelin acetate plus IM hMG and hCG for controlled ovarian 
      hyperstimulation; blood samples were collected for plasma LH and steroid 
      determinations. MAIN OUTCOME MEASURES: Presence or absence of detectable ovarian 
      11 beta-HSD activity, oocyte fertilization rates, and the identification of 
      clinical pregnancies. RESULTS: None of the 101 cycles associated with 11 beta-HSD 
      positive granulosa-lutein cells resulted in clinical pregnancies, whereas the 
      pregnancy rate for the 71 patients with undetectable ovarian 11 beta-HSD activity 
      was 63.4% per cycle. The incidence of total fertilization failure was lower and 
      the median oocyte fertilization rate was higher in cycles characterized by 11 
      beta-HSD-negative cells. Plasma concentrations of LH and E2 were higher in cycles 
      yielding 11 beta-HSD-negative cells, whereas plasma P, patient age, and the 
      number of oocytes retrieved did not relate to ovarian 11 beta-HSD activity. For 
      the 35 patients studied in repeat cycles, ovarian 11 beta-HSD activities did not 
      relate to those in the initial cycles. CONCLUSIONS: Ovarian 11 beta-HSD activity 
      is associated with failure to conceive by IVF-ET. For a given patient, ovarian 11 
      beta-HSD activity varies between consecutive treatment cycles. Hence, ovarian 11 
      beta-HSD activities may predict the outcome of this assisted reproduction 
      protocol independently in each treatment cycle.
FAU - Michael, A E
AU  - Michael AE
AD  - Department of Biochemistry and Molecular Biology, Royal Free Hospital School of 
      Medicine, University of London, United Kingdom.
FAU - Gregory, L
AU  - Gregory L
FAU - Piercy, E C
AU  - Piercy EC
FAU - Walker, S M
AU  - Walker SM
FAU - Shaw, R W
AU  - Shaw RW
FAU - Cooke, B A
AU  - Cooke BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Chorionic Gonadotropin)
RN  - 4TI98Z838E (Estradiol)
RN  - 61489-71-2 (Menotropins)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - PXW8U3YXDV (Buserelin)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Aging/metabolism
MH  - Buserelin/therapeutic use
MH  - Chorionic Gonadotropin/therapeutic use
MH  - *Embryo Transfer
MH  - Estradiol/blood
MH  - Female
MH  - *Fertilization in Vitro
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Luteinizing Hormone/blood
MH  - Menotropins/therapeutic use
MH  - Ovary/*enzymology
MH  - Pregnancy
MH  - Retrospective Studies
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - S0015-0282(16)57798-X [pii]
PST - ppublish
SO  - Fertil Steril. 1995 Sep;64(3):590-8.