PMID- 7642717
OWN - NLM
STAT- MEDLINE
DCOM- 19950915
LR  - 20131121
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 58
IP  - 1
DP  - 1995 May
TI  - Intranuclear binding of nucleoplasmin.
PG  - 105-14
AB  - Many proteins--including not only structural proteins, but also enzymes, hormone 
      receptors, and other transcription factors--accumulate to much higher nuclear 
      than cytoplasmic concentrations. Nuclear localization sequences or signals (NLSs) 
      within their primary structures entrain specific transport of these proteins 
      through the nuclear pore complexes. This transport process is energy-dependent, 
      but evidence for a true active transport mechanism is not conclusive. An 
      alternative mechanism--facilitated transport of NLS proteins followed by their 
      intranuclear binding--has been implicated by experiments with oil-isolated 
      nuclei. However, there has been no agreement as to a role for binding in the in 
      vivo nuclear accumulation of NLS-containing proteins. We demonstrate herein that 
      a prototypical NLS protein, nucleoplasmin (Np), binds within the nucleus of the 
      living Xenopus oocyte and that this binding accounts for its nuclear 
      accumulation.
FAU - Paine, P L
AU  - Paine PL
AD  - Department of Biological Sciences, St. John's University, Jamaica, New York 
      11439, USA.
FAU - Yassin, R
AU  - Yassin R
FAU - Paine, T M
AU  - Paine TM
FAU - Vancurova, I
AU  - Vancurova I
FAU - George, C
AU  - George C
FAU - Sahay, N
AU  - Sahay N
LA  - eng
GR  - GM 44390/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleoplasmins)
RN  - 0 (Phosphoproteins)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Cell Nucleus/*metabolism
MH  - Cytoplasm/metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - Microinjections
MH  - Nuclear Envelope/physiology
MH  - Nuclear Proteins/immunology/*metabolism
MH  - Nucleoplasmins
MH  - Oocytes/metabolism
MH  - *Phosphoproteins
MH  - Phosphorylation
MH  - Progesterone/pharmacology
MH  - Protein Binding
MH  - Time Factors
MH  - Xenopus laevis
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1002/jcb.240580113 [doi]
PST - ppublish
SO  - J Cell Biochem. 1995 May;58(1):105-14. doi: 10.1002/jcb.240580113.