PMID- 7646336
OWN - NLM
STAT- MEDLINE
DCOM- 19950921
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 140
IP  - 1
DP  - 1995
TI  - The role of interferon beta in human cytomegalovirus-mediated inhibition of HLA 
      DR induction on endothelial cells.
PG  - 111-26
AB  - Human cytomegalovirus (HCMV), a member of the virus family Herpesviridae that is 
      associated with extensive worldwide morbidity and mortality in immunocompromised 
      hosts, inhibits interferon-gamma (IFN gamma)-mediated induction of human 
      leukocyte antigen (HLA) class II antigens on endothelial cells. In this study, 
      the ability of HCMV-infected endothelial cells to synthesize interferon-beta (IFN 
      beta), and the role of IFN beta in HCMV-mediated inhibition of HLA class II 
      induction, was investigated. As determined by an encephalomyocarditis virus 
      protection assay, HCMV-infected endothelial cell culture supernatants contained 
      240 IU/ml of IFN type I activity, of which 99.9% was IFN beta, as compared to the 
      absence of IFN beta in mock-infected culture supernatants. UV-irradiated 
      supernatants from HCMV-infected cultures inhibited induction of HLA class II in 
      noninfected cultures by 24%. This inhibition could be abolished with 500 NU/ml of 
      anti-IFN beta antibody. Addition of anti-IFN beta antibody directly to 
      HCMV-infected cultures mitigated but did not abolish HLA class II antigen 
      inhibition. Dual immunohistochemistry for HCMV and HLA DR demonstrated that 
      infected cells, in contrast to noninfected cells, were rarely induced to express 
      HLA class II even in the presence of anti-IFN beta antibody. These findings 
      suggest that HCMV inhibits induction of HLA class II antigens by IFN beta 
      dependent and independent mechanisms.
FAU - Sedmak, D D
AU  - Sedmak DD
AD  - Department of Pathology, Ohio State University College of Medicine, Columbus, 
      USA.
FAU - Chaiwiriyakul, S
AU  - Chaiwiriyakul S
FAU - Knight, D A
AU  - Knight DA
FAU - Waldmann, W J
AU  - Waldmann WJ
LA  - eng
GR  - AI29002/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (HLA-DR Antigens)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Cells, Cultured
MH  - Cytomegalovirus/*physiology
MH  - Endothelium, Vascular/cytology/metabolism/virology
MH  - Fluorescent Antibody Technique
MH  - HLA-DR Antigens/*biosynthesis
MH  - Humans
MH  - Interferon-beta/biosynthesis/immunology/*physiology
MH  - Neutralization Tests
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF01309727 [doi]
PST - ppublish
SO  - Arch Virol. 1995;140(1):111-26. doi: 10.1007/BF01309727.