PMID- 7646363
OWN - NLM
STAT- MEDLINE
DCOM- 19950920
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 140
IP  - 7
DP  - 1995
TI  - Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of 
      porcine reproductive and respiratory syndrome virus.
PG  - 1313-9
AB  - The sequence of ORFs 2 to 4 of a U.S. isolate of porcine reproductive and 
      respiratory syndrome virus (PRRSV), ATCC VR2385, was determined by analysis of a 
      cDNA lambda library. The cDNA clones containing PRRSV specific sequences were 
      selected using a VR2385 ORF 4 specific PCR probe and sequenced. The ORFs 2, 3 and 
      4 overlapped each other and encoded polypeptides with predicted M(r) of 29.5 kDa 
      (ORF 2), 28.7 kDa (ORF 3) and 19.5 kDa (ORF 4), respectively. No overlap was 
      found between ORFs 4 and 5, and instead there was a 10 bp sequence which 
      separated these two ORFs. The nucleic acid homology with corresponding ORFs of 
      the European PRRSV isolate Lelystad virus (LV) was 65% for ORF 2, 64% for ORF 3 
      and 66% for ORF 4. Comparison of the ORF 4 sequences of VR2385 with that of 
      another U.S. isolate MN-1b revealed only 86% amino acid sequence homology and the 
      presence of deletions in the ORF 4 of MN-1b. Our results further strengthen the 
      observation that there is sequence variation between US and European PRRSV 
      isolates.
FAU - Morozov, I
AU  - Morozov I
AD  - Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State 
      University, Ames, USA.
FAU - Meng, X J
AU  - Meng XJ
FAU - Paul, P S
AU  - Paul PS
LA  - eng
SI  - GENBANK/U20788
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arterivirus/*genetics/isolation & purification
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Viral/genetics
MH  - Molecular Sequence Data
MH  - *Open Reading Frames
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Sequence Homology, Amino Acid
MH  - Swine/*virology
MH  - United States
PMC - PMC7087238
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 2020/03/23
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - BF01322758 [pii]
AID - 10.1007/BF01322758 [doi]
PST - ppublish
SO  - Arch Virol. 1995;140(7):1313-9. doi: 10.1007/BF01322758.