PMID- 7647013
OWN - NLM
STAT- MEDLINE
DCOM- 19950925
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 90
IP  - 3
DP  - 1995 Jul
TI  - Combined immunophenotyping and in situ hybridization (FICTION): a rapid method to 
      study cell lineage involvement in myelodysplastic syndromes.
PG  - 701-6
AB  - We present a study in which we used a recently described method combining 
      fluorescence in situ hybridization (FISH) and immunophenotyping, i.e. FICTION, to 
      assess the involvement of different cell lineages in myelodysplastic syndrome 
      (MDS) with monosomy 7 (-7), trisomy 8 (+8) or loss of Y chromosome (-Y). Blood or 
      marrow smears or cytocentrifuge preparations were stained both by antibodies to 
      granulocytes (CD15), monocytes (CD14), T lymphocytes (CD3), B lymphocytes (CD20) 
      and by probes specific for chromosomes 7, 8 or Y. Of nine cases of MDS with -7, 
      four with +8 and two with -Y studied, none showed lymphocytic involvement by the 
      chromosome abnormality. In contrast, -7, +8 and -Y were found in granulocytes and 
      monocytes in all patients studied, but they involved a variable proportion of 
      those cells. The partial involvement by -7 and +8 seen in some cases suggests 
      that myelopoiesis was only partially clonal in those cases, or that the 
      chromosome abnormality was a secondary event in the MDS process. FICTION 
      therefore appears to be a simple and easily reproducible method that can be used 
      for the assessment of lineage involvement in MDS and other haematological 
      malignancies.
FAU - Soenen, V
AU  - Soenen V
AD  - Service d'Hematologie A, C.H.U. Lille, France.
FAU - Fenaux, P
AU  - Fenaux P
FAU - Flactif, M
AU  - Flactif M
FAU - Lepelley, P
AU  - Lepelley P
FAU - Lai, J L
AU  - Lai JL
FAU - Cosson, A
AU  - Cosson A
FAU - Preudhomme, C
AU  - Preudhomme C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - B-Lymphocytes/pathology
MH  - *Chromosome Aberrations
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Granulocytes/pathology
MH  - Hematopoietic Stem Cells/pathology
MH  - Humans
MH  - *Immunophenotyping
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Monocytes/pathology
MH  - Myelodysplastic Syndromes/*genetics/*pathology
MH  - T-Lymphocytes/pathology
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1995.tb05604.x [doi]
PST - ppublish
SO  - Br J Haematol. 1995 Jul;90(3):701-6. doi: 10.1111/j.1365-2141.1995.tb05604.x.