PMID- 7647464
OWN - NLM
STAT- MEDLINE
DCOM- 19950926
LR  - 20190904
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 6
IP  - 6
DP  - 1995 Jun
TI  - The bovine bivariate flow karyotype and peak identification by chromosome 
      painting with PCR-generated probes.
PG  - 415-20
AB  - A bovine bivariate flow karyotype has been established from a primary fibroblast 
      cell culture carrying a 4;10 Robertsonian translocation. From 27 to 36 
      populations could be resolved by flow cytometry although the anticipated number 
      was 31. Separation of chromosomal pairs into two populations explains this high 
      resolution and confirms the high level of heteromorphism previously observed. We 
      used a PARM-PCR (Priming Authorizing Random Mismatches) procedure for the 
      production of paint probes from flow-sorted chromosome fractions. These probes 
      were used for chromosome identification by fluorescence in situ hybridization 
      (FISH) on R-banded metaphase spreads. We present the localization of all the 
      bovine chromosome types on the flow karyotype. Twenty-two chromosome types 
      including the translocated chromosome were sorted as pure fractions.
FAU - Schmitz, A
AU  - Schmitz A
AD  - Centre d'etudes nucleaires de Fontenay aux roses (CEA), Direction des Sciences du 
      vivant, Laboratoire de Cytometrie, France.
FAU - Oustry, A
AU  - Oustry A
FAU - Chaput, B
AU  - Chaput B
FAU - Bahri-Darwich, I
AU  - Bahri-Darwich I
FAU - Yerle, M
AU  - Yerle M
FAU - Millan, D
AU  - Millan D
FAU - Frelat, G
AU  - Frelat G
FAU - Cribiu, E P
AU  - Cribiu EP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle/*genetics
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Fibroblasts/cytology
MH  - Flow Cytometry/methods/*veterinary
MH  - Karyotyping/methods/*veterinary
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/veterinary
MH  - Species Specificity
MH  - Translocation, Genetic
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1007/BF00355643 [doi]
PST - ppublish
SO  - Mamm Genome. 1995 Jun;6(6):415-20. doi: 10.1007/BF00355643.