PMID- 7647559
OWN - NLM
STAT- MEDLINE
DCOM- 19950922
LR  - 20190920
IS  - 0925-2738 (Print)
IS  - 0925-2738 (Linking)
VI  - 5
IP  - 4
DP  - 1995 Jun
TI  - Probing internal water molecules in proteins using two-dimensional 19F-1H NMR.
PG  - 415-9
AB  - A simple approach for detecting internal water molecules in proteins in solution 
      is described. This approach combines 19F-detected heteronuclear Overhauser and 
      exchange spectroscopy (HOESY) with site-specific 19F substitution. The model 
      system employed was intestinal fatty acid-binding protein complexed with 
      [2-mono-19F]-palmitate. An intense cross peak was observed between the fluorine 
      and a buried water molecule, as defined in the 1.98 A crystal structure of the 
      complex. From HOESY spectra, the fluorine-water distance was estimated to be 2.1 
      A, in agreement with the crystal structure. This approach may be applicable to 
      macromolecules that are too large for 1H-detected NMR methods.
FAU - Cistola, D P
AU  - Cistola DP
AD  - Department of Biochemistry and Molecular Biophysics, Washington University School 
      of Medicine, St. Louis, MO 63110-1093, USA.
FAU - Hall, K B
AU  - Hall KB
LA  - eng
GR  - GM46318/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Biomol NMR
JT  - Journal of biomolecular NMR
JID - 9110829
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp7 protein, rat)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proteins)
RN  - 0 (Solutions)
RN  - 059QF0KO0R (Water)
RN  - 284SYP0193 (Fluorine)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Carrier Proteins/chemistry
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Fatty Acids/metabolism
MH  - Fluorine/chemistry
MH  - Hydrogen/chemistry
MH  - Macromolecular Substances
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Models, Molecular
MH  - Molecular Structure
MH  - *Neoplasm Proteins
MH  - *Nerve Tissue Proteins
MH  - Proteins/*chemistry
MH  - Rats
MH  - Solutions
MH  - Water/*chemistry
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1007/BF00182285 [doi]
PST - ppublish
SO  - J Biomol NMR. 1995 Jun;5(4):415-9. doi: 10.1007/BF00182285.