PMID- 7648381
OWN - NLM
STAT- MEDLINE
DCOM- 19950928
LR  - 20081121
IS  - 1081-1206 (Print)
IS  - 1081-1206 (Linking)
VI  - 75
IP  - 2
DP  - 1995 Aug
TI  - IFN-gamma activates superoxide anion production in blood monocytes from allergic 
      asthmatic patients.
PG  - 162-6
AB  - BACKGROUND: Peripheral blood monocytes may participate in allergic diseases by 
      releasing inflammatory mediators. We have recently shown enhancement of 
      nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase activity in 
      adherent human peripheral blood monocytes from allergic patients when 
      immunoglobulin E (IgE) binds to its low affinity receptor. Interferon-gamma 
      (IFN-gamma), a monocyte-activating lymphokine, has been shown to prime monocytes 
      for superoxide anion release. OBJECTIVE: We hypothesized that IFN-gamma could 
      directly activate blood monocyte superoxide anion release and evaluated its 
      modulatory effect on IgE-induced superoxide anion release from those cells. 
      METHODS: Seven patients with allergic asthma, 6 patients with allergic rhinitis, 
      and five nonallergic controls were studied. We measured superoxide anion release 
      from their blood monocytes using a chemiluminescence assay. Cells were either 
      nonstimulated, or stimulated for 30 minutes with IFN-gamma and/or serum IgE. 
      RESULTS: We found that IFN-gamma stimulated superoxide anion production and 
      decreased the IgE-induced superoxide anion production after 30 minutes of 
      IFN-gamma preincubation only in blood monocytes from patients with allergic 
      asthma. CONCLUSIONS: These data suggest that IFN-gamma receptors may be increased 
      and/or coupled to NADPH oxidase in allergic asthmatic patients.
FAU - Demoly, P
AU  - Demoly P
AD  - Clinique des Maladies Respiratoires and INSERM CJF 92-10, Hopital Arnaud de 
      Villeneuve, Centre Hospitalier Universitaire, Montpellier, France.
FAU - Damon, M
AU  - Damon M
FAU - Michel, F B
AU  - Michel FB
FAU - Godard, P
AU  - Godard P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Allergy Asthma Immunol
JT  - Annals of allergy, asthma & immunology : official publication of the American 
      College of Allergy, Asthma, & Immunology
JID - 9503580
RN  - 11062-77-4 (Superoxides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Asthma/*blood
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Interferon-gamma/*pharmacology
MH  - Male
MH  - Monocytes/*metabolism
MH  - Rhinitis, Allergic, Perennial/blood
MH  - Superoxides/*metabolism
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
PST - ppublish
SO  - Ann Allergy Asthma Immunol. 1995 Aug;75(2):162-6.