PMID- 7649123
OWN - NLM
STAT- MEDLINE
DCOM- 19950922
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 136
IP  - 9
DP  - 1995 Sep
TI  - Differential gene expression of growth hormone (GH)-releasing hormone (GRH) and 
      GRH receptor in various rat tissues.
PG  - 4147-50
AB  - Growth hormone (GH)-releasing hormone (GRH) acts on specific receptors in the 
      anterior pituitary to stimulate the synthesis and release of GH. Recent reports 
      suggest that GRH is also synthesized in extrahypothalamic tissues. To evaluate 
      the potential roles of extrahypothalamic GRH, we studied the gene expression of 
      GRH and GRH receptors in various rat tissues by reverse transcribed 
      (RT)-polymerase chain reaction (PCR). Total RNA was extracted from twenty-three 
      rat organs and RT-PCR was performed with GRH and GRH receptor primers. 
      Highly-sensitive RT-PCR-Southern blotting showed that GRH and GRH receptor mRNA 
      coexist in the widespread tissues (14 of 25 tissues). GRH mRNA was relatively 
      abundant in the cerebral cortex, brain stem, testis, and placenta, while GRH 
      receptor mRNA was abundant in renal medulla and renal pelvis. Northern blot 
      hybridization using poly A+ RNA indicated that the transcript of GRH receptor 
      gene found in the renal medulla was similar to the longer transcript (about 4 Kb) 
      of pituitary GRH receptor in the size. These results suggest that GRH plays a 
      potential role not only in the neuroendocrine axis, but also in the autocrine and 
      paracrine systems in extrahypothalamic tissues.
FAU - Matsubara, S
AU  - Matsubara S
AD  - First Department of Internal Medicine, Kagawa Medical School, Japan.
FAU - Sato, M
AU  - Sato M
FAU - Mizobuchi, M
AU  - Mizobuchi M
FAU - Niimi, M
AU  - Niimi M
FAU - Takahara, J
AU  - Takahara J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (Receptors, Pituitary Hormone-Regulating Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
RN  - F8L0ODC9D7 (somatotropin releasing hormone receptor)
SB  - IM
GS  - GRH
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Brain/metabolism/ultrastructure
MH  - *Brain Chemistry
MH  - Brain Stem/chemistry/metabolism/ultrastructure
MH  - Cerebral Cortex/chemistry/metabolism/ultrastructure
MH  - Female
MH  - Gene Expression
MH  - Growth Hormone-Releasing Hormone/analysis/*genetics/metabolism
MH  - Hypothalamus/chemistry/metabolism/ultrastructure
MH  - Kidney/*chemistry/metabolism/ultrastructure
MH  - Male
MH  - Molecular Sequence Data
MH  - Placenta/*chemistry/metabolism/ultrastructure
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - RNA, Messenger/analysis/chemistry/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Neuropeptide/analysis/*genetics/metabolism
MH  - Receptors, Pituitary Hormone-Regulating Hormone/analysis/*genetics/metabolism
MH  - Testis/*chemistry/metabolism/ultrastructure
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1210/endo.136.9.7649123 [doi]
PST - ppublish
SO  - Endocrinology. 1995 Sep;136(9):4147-50. doi: 10.1210/endo.136.9.7649123.