PMID- 7649566
OWN - NLM
STAT- MEDLINE
DCOM- 19950926
LR  - 20131121
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 192
IP  - 5
DP  - 1995 Apr
TI  - Changes of intracellular free calcium concentration in human polymorphonuclear 
      leukocytes after repeated stimulations with 
      N-formyl-methionyl-leucyl-phenylalanine.
PG  - 343-52
AB  - A rapid transient rise in the intracellular free calcium concentration ( Ca2+]i) 
      is an important step in human polymorphonuclear leukocytes (PMNL) activation. 
      This can be caused by many inflammatory mediators and has been implicated in the 
      regulation of various cellular reactions. In this study we investigated the 
      changes of [Ca2+]i in human PMNL activated three times with 10(-7)M 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP). PMNL in the presence of 1 mM Ca2+ 
      were able to respond to three consecutive stimulations with FMLP. The first Ca2+ 
      response was the highest one and was a result of Ca2+ release from internal 
      stores (which was responsible for about 30% of maximal increment in [Ca2+]i) and 
      the extracellular Ca2+ influx. Experiments with PMNL suspended in a medium 
      containing 100 nM Ca2+ and pretreated with 1 nM Ni2+ (an inorganic calcium 
      channel blocker) revealed that the second and third response is completely 
      dependent on the extracellular Ca2+ influx. Changes of the time interval between 
      stimulations had no influence on the occurrence of extracellular Ca2+ influx 
      related to second addition of FMLP. Elongation of the time interval up to 30 min 
      did not restore the release of Ca2+ from internal stores. It indicates the 
      occurrence of dissociation of Ca2+ release from intracellular stores and 
      extracellular Ca2+ influx during the second and third PMNL response to FMLP.
FAU - Nowak, D
AU  - Nowak D
AD  - Department of Pneumonology and Allergology, Medical University of Lodz, Poland.
FAU - Bialasiewicz, P
AU  - Bialasiewicz P
FAU - Antczak, A
AU  - Antczak A
FAU - Krol, M
AU  - Krol M
FAU - Piasecka, G
AU  - Piasecka G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*analysis
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*drug effects/*metabolism
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - S0171-2985(11)80174-9 [pii]
AID - 10.1016/S0171-2985(11)80174-9 [doi]
PST - ppublish
SO  - Immunobiology. 1995 Apr;192(5):343-52. doi: 10.1016/S0171-2985(11)80174-9.