PMID- 7653221
OWN - NLM
STAT- MEDLINE
DCOM- 19950926
LR  - 20141120
IS  - 0001-5814 (Print)
IS  - 0001-5814 (Linking)
VI  - 26
IP  - 2
DP  - 1995
TI  - Effects of human neutrophil elastase and cathepsin G on the reactivity of 
      platelets with antiplatelet antibodies.
PG  - 163-70
AB  - Two human neutrophil serine proteases, elastase (HNE) and cathepsin G (CathG), 
      are known to change the structure and hemostatic function of platelet surface 
      membrane. The platelet membrane contains glycoproteins (GPs) which function as 
      alloantigens, autoantigens and targets of drug-induced antibodies. The aim of 
      this study was to investigate whether proteolysis of platelet GPs by HNE and 
      CathG is associated with changes in the reactivity of platelets to antiplatelet 
      antibodies. The platelet immunoreactivity was examined using the MAIPA 
      (monoclonal antibody-specific immobilization of platelet antigens) assay and 
      PSIFT (platelet suspension immunofluorescence test). The treatment of platelets 
      with HNE led to a moderate increase in their reactivity to quinidine-dependent 
      (anti-GP Ib) antibody and to a slight decline in the expression of HPA-1a. In 
      contrast, CathG did not provoke any significant changes in platelet reactions 
      with quinidine dependent and anti-HPA-1a antibodies. Both enzymes had no 
      significant effect on the expression of HLA-A2, HLA-A3, HLA-B7 and HLA-B8 on 
      platelets.
FAU - Bykowska, K
AU  - Bykowska K
AD  - Laboratory or Blood Coagulation and Hemostasis, Institute of Hematology and Blood 
      Transfusion, Warsaw.
FAU - Maslanka, K
AU  - Maslanka K
FAU - Uhrynowska, M
AU  - Uhrynowska M
FAU - Kopec, M
AU  - Kopec M
FAU - Lopaciuk, S
AU  - Lopaciuk S
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Acta Haematol Pol
JT  - Acta haematologica Polonica
JID - 0262610
RN  - 0 (Antibodies)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.20 (CTSG protein, human)
RN  - EC 3.4.21.20 (Cathepsin G)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
SB  - IM
MH  - Antibodies/*immunology
MH  - Blood Platelets/*immunology
MH  - Cathepsin G
MH  - Cathepsins/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoblotting
MH  - In Vitro Techniques
MH  - Leukocyte Elastase
MH  - Pancreatic Elastase/*metabolism
MH  - Platelet Membrane Glycoproteins/*metabolism
MH  - Serine Endopeptidases/*metabolism
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - Acta Haematol Pol. 1995;26(2):163-70.