PMID- 7656204
OWN - NLM
STAT- MEDLINE
DCOM- 19951004
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 83
IP  - 1
DP  - 1995 Aug
TI  - Detection of 9p deletions in leukemia cell lines by interphase fluorescence in 
      situ hybridization with YAC-derived probes.
PG  - 46-55
AB  - Hemizygous and homozygous deletions of the type I interferon gene cluster (IFN) 
      have been detected in about 20% of acute lymphoblastic leukemias. A putative 
      tumor suppressor gene (TSG) is thought to be located centromeric to the IFN 
      cluster on chromosomal bands 9p21-22. We studied the accuracy of fluorescence in 
      situ hybridization (FISH) for detecting deletions in interphase cells using yeast 
      artificial chromosome (YAC) clones containing all or part of the IFN cluster. 
      FISH probes were generated from YACs (320-1300 kb in size) by a 
      sequence-independent amplification technique (SIA). Fifteen cell lines (nine 
      T-ALL, three B-cell precursor ALL, one B-ALL, one AML, one CML-BC) that had been 
      well characterized by conventional cytogenetic analysis and molecular techniques 
      were analyzed. We were able to detect all numerical changes of the IFN cluster 
      including homozygous and hemizygous deletions accurately and to define subclones 
      of the cell lines. Moreover, in six cell lines we were able to identify 
      subclones. In dilution experiments the detection thresholds for subpopulations 
      with homozygous and hemizygous deletions were determined to be 5% and 7.5%, 
      respectively.
FAU - Dreyling, M H
AU  - Dreyling MH
AD  - Department of Medicine, University of Chicago, Illinois 60637, USA.
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Olopade, O I
AU  - Olopade OI
FAU - Le Beau, M M
AU  - Le Beau MM
FAU - Rowley, J D
AU  - Rowley JD
FAU - Bohlander, S K
AU  - Bohlander SK
LA  - eng
GR  - CA42557/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - Base Sequence
MH  - *Chromosome Deletion
MH  - Chromosomes, Artificial, Yeast
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/*genetics
MH  - Metaphase
MH  - Molecular Sequence Data
MH  - Ploidies
MH  - Sensitivity and Specificity
MH  - Tumor Cells, Cultured
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - S0165460895000267 [pii]
AID - 10.1016/s0165-4608(95)00026-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Aug;83(1):46-55. doi: 10.1016/s0165-4608(95)00026-7.