PMID- 7657294
OWN - NLM
STAT- MEDLINE
DCOM- 19951004
LR  - 20071114
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 22
IP  - 3
DP  - 1995 Sep
TI  - Differential expression and release of CD54 induced by cytokines.
PG  - 866-75
AB  - Intercellular adhesion molecule-1 (ICAM-1, CD54) is upregulated in many cell 
      types stimulated by cytokines. A human hepatoblastoma cell line (C3A, a subclone 
      of HepG2/C3 that is currently being used as a surrogate liver) and human lung 
      adenocarcinoma cells (A549) were stimulated with interleukin-1 beta (IL-1 beta), 
      tumor necrosis factor-alpha (TNF alpha), interferon-gamma (IFN gamma), or IL-6 to 
      determine any differences in cell type responsiveness to individual cytokines for 
      ICAM-1 upregulation. Time courses were performed with each cytokine evaluating 
      ICAM-1 mRNA, surface expression, and cICAM-1 in the cell culture media. Between 3 
      and 6 hours, IL-1 beta (30 U/mL) stimulated the greatest increase in hepatocyte 
      ICAM-1 mRNA, followed by IFN gamma (100 U/ mL), and IL-6 (100 U/mL) in order of 
      potency. Except for IL-6, cytokine-induced hepatocyte surface levels of ICAM-1 
      (immunofluorescence flow cytometry, mAb R6.5) were dose dependent, with 
      inhibition at higher concentration. Highest levels followed stimulation with INF 
      gamma (P < .05). Significantly less was found after both IL-1 beta and TNF alpha; 
      none was detected after IL-6 (P < .05). In contrast, IL-1 beta stimulated 
      significantly more cICAM-1 release from hepatocytes than the other cytokines (P < 
      .001), and IL-6 stimulated modest cICAM-1. Between 3 and 6 hours in the A549 
      cells, IL-1 beta stimulated the greatest increase in ICAM-1 mRNA, followed by TNF 
      alpha. Both responses were greater than that observed in the hepatocytes. IFN 
      gamma- and IL-6-induced ICAM-1 mRNA synthesis was not different from unstimulated 
      A549 cells. Cytokine-induced A549 surface levels of ICAM-1 (immunofluorescence 
      flow cytometry, mAb R6.5) was highest for IL-1 beta (peak levels similar to 
      hepatocyte response), modest with TNF alpha (peak levels less than hepatocytes), 
      detectable with IFN gamma (much less than hepatocytes), and nondetectable after 
      IL-6. No ICAM-1 release from A549 cells was induced under any condition. In 
      hepatocytes the amount of ICAM-1 mRNA was best accounted for by considering both 
      cell surface levels of ICAM-1 and cICAM-1 levels. In human lung adenocarcinoma 
      cells, the cytokine induction of ICAM-1 mRNA could potentially be accounted for 
      by observing cell surface levels of ICAM-1 because no cICAM-1 was produced. These 
      results suggest that surface ICAM-1 and cICAM-1 may be differentially controlled 
      by each cytokine and by each parenchymal cell type.
FAU - Mickelson, J K
AU  - Mickelson JK
AD  - Section of Cardiology, Methodist Hospital, Houston TX, USA.
FAU - Kukielka, G
AU  - Kukielka G
FAU - Bravenec, J S
AU  - Bravenec JS
FAU - Mainolfi, E
AU  - Mainolfi E
FAU - Rothlein, R
AU  - Rothlein R
FAU - Hawkins, H K
AU  - Hawkins HK
FAU - Kelly, J H
AU  - Kelly JH
FAU - Smith, C W
AU  - Smith CW
LA  - eng
GR  - ES06091/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Adenocarcinoma/metabolism/pathology
MH  - Culture Media/metabolism
MH  - Cytokines/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Hepatoblastoma/metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - Liver Neoplasms/metabolism/pathology
MH  - Lung Neoplasms/metabolism/pathology
MH  - RNA, Messenger/metabolism
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 0270-9139(95)90309-7 [pii]
PST - ppublish
SO  - Hepatology. 1995 Sep;22(3):866-75.